Measurement of Immunoglobulin Synthesis Using the ELISPOT Assay

Various methods have been devised to detect antibody-producing cells in culture and also determine the isotype of the immunoglobulin produced. We have found the ELISPOT assay to be the method of choice for detecting human monoclonal antibodies (MAbs) produced by heterohybridomas (see Chapter 4 ) (1 ). The method used in our laboratory to detect and isotype human MAb specific to bacterial antigens is described here.