ACIDIC-NATIVE GEL Protocol( For Basic Proteins (pI7.0))
USE ONLY FRESH GELSStock Solutions1) 1.5M Acetate-KOH pH 4.3 (48ml 1MKOH + 17.2m
USE ONLY FRESH GELS
Stock Solutions
1) 1.5M Acetate-KOH pH 4.3 (48ml 1MKOH + 17.2ml AcH + H2O up to 200ml) - Keep RT. 2) 30% Acrylamide 0.8% Methylene bis Acrylamide. Keep 4°C. 3) 0.25M Acetate-KOH pH 6.8 (48ml 1MKOH + 2.9ml AcH + H2O up to 200ml) - Keep RT. 4) 10% Ammonium Persulfate (APS). Keep 4°C less than 1 month.
Running Buffer x1
| ß-Alanine | 0.35M | 18.7gr |
| AcH | 0.14M | 4.8ml |
| H2O up to | 600ml | |
| Adjust pH to | 4.3 |
Keep at RT
Dissolving Buffer x5
| Glycerol 50% | 1.45ml |
| 0.25M Acetate-KOH pH 6.8 | 0.5ml |
| Methyl Green | traces |
Keep in aliquots of 1ml at -20C
相关文章
