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Primer-Directed Site-Specific Mutagenesis
Site-directed mutagenesis provides a powerful means for probing protein structur
2025-03-27 57 -
Using Single-Strand Conformational Polymorphism Gel Electrophoresis to Analyze Mutually Exclusive Al
Single-strand conformational polymorphism analysis has been used successfully to
2025-03-27 85 -
Single Enzyme Studies: A Historical Perspective
Single-molecule enzymology has a longer history than is often supposed, with the
2025-03-27 79 -
DNA-Templated Native Chemical Ligation of Functionalized Peptide Nucleic Acids: A Versatile Tool for
Single base-specific detection of DNA/RNA sequences is of importance in the diag
2025-03-27 84 -
Ligation of DNA with T4 DNA Ligase
Since they are involved in such important processes as DNA replication, DNA repa
2025-03-27 86 -
Yeast Artificial Chromosomes
Since their mtroductlon in 1987, yeast artificial chromosomes (commonly referred
2025-03-27 80 -
Separation of Enantiomers by Capillary Electrophoresis Using Cyclodextrins
Since the pioneering publication of first paper on application of capillary elec
2025-03-27 80 -
Using a Noncovalent Protection Strategy to Enhance Solid-Phase Synthesis
Since the introduction of solid-phase peptide synthesis by Merrifield (1 ) nearl
2025-03-27 82 -
Weak Affinity Chromatography
Since the inception of affinity chromatography 30 years ago (1 ), it has develop
2025-03-27 82 -
Inverse Polymerase Chain Reaction
Since the first report on cDNA cloning in 1972 (1 ), this technology has been de
2025-03-27 79 -
Development of a High-Throughput Capillary Electrophoresis Protocol for DNA Fragment Analysis
Since the first descriptions of electrophoresis in small diameter tubes in the 1
2025-03-27 80 -
Production of Panels of Monoclonal Antibodies by the Hybridoma Method
Since the first description by Kohler and Milstein (1 ), many variations on this
2025-03-27 80 -
Developing a Minimally Immunogenic Humanized Antibody by SDR Grafting
Since the advent of hybridoma technology a quarter century ago, a large number o
2025-03-27 66 -
Mapping of Signaling Pathways by Functional Interaction Proteomics
Signaling pathways transduce extracellular stimuli from the membrane to the nucl
2025-03-27 87 -
General Consideration on Sialic Acid Chemistry
Sialic acids, also known as neuraminic acids, are a family of negatively charged
2025-03-27 81
