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  • Isolation of High Molecular Weight DNA Suitable for the Construction of Genomic Libraries

    Isolation of High Molecular Weight DNA Suitable for the Construction of Genomic Libraries

    Recent advances in molecular biology have made it possible to construct complete

    2025-03-27 60
  • Purification of Water-Soluble Natural Products

    Purification of Water-Soluble Natural Products

    Recent advancement in separation and structure elucidation methods has been rema

    2025-03-27 84
  • TaqMan Reverse Transcriptase-Polymerase Chain Reaction Coupled With Capillary Electrophoresis for Qu

    TaqMan Reverse Transcriptase-Polymerase Chain Reaction Coupled With Capillary Electrophoresis for Qu

    Real-time TaqMan� polymerase chain reaction (PCR) assays allow quantification of

    2025-03-27 87
  • Rapid and simple comparison of messenger rna levels using real-time PCR

    Rapid and simple comparison of messenger rna levels using real-time PCR

    Real-time polymerase chain reaction (PCR) constitutes a significant improvement

    2025-03-27 73
  • Real-Time Monitoring the Spatiotemporal Dynamics of Intracellular cGMP in Vascular Smooth Muscle Cel

    Real-Time Monitoring the Spatiotemporal Dynamics of Intracellular cGMP in Vascular Smooth Muscle Cel

    Real-time and noninvasive imaging of intracellular second messengers in mammalia

    2025-03-27 59
  • In vitro Sphingomyelinase Assay

    In vitro Sphingomyelinase Assay

    Reagents: Lysis buffer 25 mM Tris-HCl, pH 7.4 5 mM EDTA 1 mM ATP 20 µg/ml CLAP 1

    2025-03-27 86
  • Indirect Immunofluorescence

    Indirect Immunofluorescence

    Reagents: NGS-Normal Goat Serum PBST-0.5% Tween-20/CMF-PBS CMF-PBS 1Ab-mouse

    2025-03-27 76
  • Phosphate Assay by Suprya Jaydev

    Phosphate Assay by Suprya Jaydev

    Reagents Ashing buffer: 10 g Mg(NO3)2 100 ml EtOH 1.5 N HCl stock: 119.7 ml conc

    2025-03-27 69
  • DGK Membrane Preparation

    DGK Membrane Preparation

    Reagents : Bacterial strain E. coli N4830/pJW10 LB amp media 50 µg/ml ampicillin

    2025-03-27 88
  • Protein A Purification of Antibody

    Protein A Purification of Antibody

    Reagents Affi-gel Protein-A Agarose (BioRad #153-6153) MAPS II Binding Buffer

    2025-03-27 75
  • Sensitive and Specific Fluorescent Probing of Oxidative Stress in Different Classes of Membrane Phos

    Sensitive and Specific Fluorescent Probing of Oxidative Stress in Different Classes of Membrane Phos

    Reactive-oxygen species and organic free radicals are essential metabolic interm

    2025-03-27 82
  • Electron Paramagnetic Resonance Oximetry and Redoximetry

    Electron Paramagnetic Resonance Oximetry and Redoximetry

    Reactive oxygen/nitrogen species (ROS/RNS) have been increasingly recognized as

    2025-03-27 76
  • Assessment of Antioxidant Activity of Eugenol In Vitro and In Vivo

    Assessment of Antioxidant Activity of Eugenol In Vitro and In Vivo

    Reactive oxygen species are implicated in many human diseases and aging process.

    2025-03-27 93
  • 常用实验动物全身性麻醉药物的使用

    常用实验动物全身性麻醉药物的使用

    卢 晓1,2, 于灵芝3, 周聪颖4, 李如颖1, 陈文君1, 江善祥2(1. 迪哲医药有限公司, 上海 201203; 2. 南京农业大学动物医学院, 南

    2025-03-26 839
  • 三溴乙醇(阿佛丁)麻醉剂问题解答(一)

    三溴乙醇(阿佛丁)麻醉剂问题解答(一)

    1.名称三溴乙醇又名阿佛丁是一种新型麻醉剂。2.浓度大鼠三溴乙醇麻醉剂和小鼠三溴乙醇麻醉剂是两款不同的产品,浓度也大不相同。通常大鼠麻醉剂浓度为2.5%;小鼠麻

    2025-03-25 807