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293-T细胞培养实验中注意事项
相关专题培养293细胞 应注意以下几个方面：1.用1640加10%胎牛血清，有的种系用DMEM .血清要求质量较高，在普通血清中细胞生长不太好。1640用双蒸水
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Ribonuclease Protection Analysis of Gene Expression in Xenopus
When characterizing the developmental expression of a novel gene, or when examin
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Observational Study Design
Much can be learned about a process by observing changes over time or by compari
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Cell Migration Analyses Within Fibroblast-Derived 3-D Matrices
Research in cell biology often is based in tissue culturing cells on artificial
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Immobilization of Enzymes on Thermo-Responsive Polymers
Immobilization of enzymes on supports is a valuable technique for increasing tot
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Analysis of Rho GTPase-Induced Localization of Nanoscale Adhesions Using Fluorescence Nanoscopy
Rho GTPases are important regulators of the formation of focal adhesions and foc
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Maintenance of Cell Culture
Maintenance of Cell CultureAuthor: Nanci DonackiSource: Contributed by Nanci Don
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Cell Lineage Analysis: X-Inactivation Mosaics
In female mammals, one of the two X chromosomes in each and every embryonic cell
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Targeting Repair Proteins to the Mitochondria of Mammalian Cells Through Stable Transfection, Transi
The mitochondrial genome represents a target for exogenous and endogenous damage
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Electroporation of Antigen-Presenting Cells for T-Cell Recognition and Cytotoxic T-Lymphocyte Primin
Specific cytotoxic T-lymphocytes (CTL) are important in the protection against v
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Cell Grafting and Fate Mapping of the Early-Somite-Stage Mouse Embryo
The development of techniques for culturing postimplantation mouse embryos has o
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Renal and Cardiac Na+K+-ATPase and Aconitase in a Rat Model of Fetal Programming
Fetal programming of adult disease is an area of research that has gained consid
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酵母菌落直接质粒转化法
酵母菌落直接质粒转化法 1． 用牙签从平板中挑取单菌落（直径 2 ～ 3mm ），转移到 1.5ml 的无菌离心管中。2． 将 10 μ l 载体 DNA （
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Generating Tamoxifen-Inducible Cre Alleles to Investigate Myogenesis in Mice
Gene inactivation has become the gold standard for determining gene function in
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G418筛选慢病毒感染稳定细胞株
用慢病毒筛选稳定细胞株，以G418筛选方法为例，筛选出稳定整合Neomycin抗性基因的细胞系。A 、 G418 抗生素最优浓度筛选每种细胞对抗生素的敏感性不同
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