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外源基因在真核细胞表达步骤
在真核细胞中表达蛋白的步骤比大肠杆菌复杂得多。首先要考虑选用什么载体。一般人们趋向于使用分泌表达载体，以便于蛋白的纯化。但有的蛋白并不适合于分泌表达，如一些胞质
2025-03-21 99 -
sf9，空斑试验，滴度测定
【求助】sf9，空斑试验，滴度测定参与者：xiaoniziwendeng我用的是bac-to-bac系统表达的目的蛋白。加上荧光底物后，96孔板读数，野生病毒和
2025-03-21 54 -
Lung Cancer and Exposure to Metals: The Epidemiological Evidence
Exposure to metallic compounds is ubiquitous, with its widespread use in industr
2025-03-21 67 -
Epigenetic Markers of Early Tumor Development
Cancer patients’ outcome and survival depends on the early diagnosis of malignan
2025-03-21 59 -
Generation of p53 Target Database Via Integration of Microarray and Global p53 DNA-Binding Site Anal
The completion of the human genome sequence and availability of cDNA microarray
2025-03-21 62 -
Collagen Invasion Assay
Invasion occurs when invasion promoter molecules outbalance the function of inva
2025-03-21 60 -
用于细胞分析的机器视觉系统简介
1996年，美国盐湖域的阿鲁普实验室遇到一个问题。该实验室从事帕帕尼科拉乌涂片的筛分工作以便找出宫颈癌症兆。当涂片上有少量异常细胞存在而又未被探测出来时，他们会
2025-03-21 64 -
Use of Splicing Reporter Minigene Assay to Evaluate the Effect on Splicing of Unclassified Genetic V
The interpretation of the numerous sequence variants of unknown biological and c
2025-03-21 58 -
G-Rich Oligonucleotides for Cancer Treatment
Oligonucleotides with guanosine-rich (G-rich) sequences often have unusual physi
2025-03-21 58 -
Construction and Characterization of Minibodies for Imaging and Therapy of Colorectal Carcinomas
Engineering of antibodies specific for tumor-associated antigens provides great
2025-03-21 69 -
软琼脂克隆形成实验
原理：细胞接种存活率只表示接种细胞后贴壁的细胞数，但贴壁后的细胞不一定每个都能增殖和形成克隆。而形成克隆的细胞必为贴壁和有增殖活力的细胞。克隆形成率反映细胞群体
2025-03-21 90 -
真核生物翻译的调控
原核生物基因表达的调控主要在转录水平上进行，而真核生物由于RNA较为稳定，所以除了存在转录水平的调控以外，在翻译水平上也进行各种形式的调控。在蛋白质生物合成的起
2025-03-21 103 -
d-Glucaric Acid as a Prospective Tumor Marker
d -Glucaric acid (GA) 1s a natural, apparently nontoxic compound produced in sma
2025-03-21 60 -
Diet, Epigenetics, and Cancer
Cancer encompasses a highly heterogeneous group of diseases. It has been thought
2025-03-21 57 -
Interaction of p53 with Cellular Proteins
Cellular proteins that interact with p53 play a major role in both positive and
2025-03-21 62
