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受精作用(fertillization)
动物的配子发生产生了两种类型的单倍体细胞:精子和卵子, 精子与卵子的融合,即受精作用,重新形成一个二倍体的细胞,即受精卵。在哺乳动物、鸟类、爬行动物,受精作用发
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细胞质膜(plasma membrane)
指包围在细胞表面的一层极薄的膜,主要由膜脂和膜蛋白所组成。质膜的基本作用是维护细胞内微环境的相对稳定,并参与同外界环境进行物质交换、能量和信息传递。另外, 在细
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通过细胞计数对疾病模型小鼠进行免疫监测
摘要 生物样本往往是非单一细胞的混合物,如原代分离的细胞样本和许多培养的细胞样本都是由不同种类或不同功能的细胞组成。而对于样本中不同细胞亚群的区分和定量恰恰是很
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Time-Resolved NMR Spectroscopy: Ligand-Induced Refolding of Riboswitches
A detailed understanding of cellular mechanisms requires knowledge of structure
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CD34阳性细胞计数
外周血现已被广泛地被用作骨髓移植、癌症患者接受大剂量化疗或放疗后骨髓重建造所需血干细胞( Hemotopoietic Progenitor Cells HPC
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Methylation-Sensitive Polymerase Chain Reaction
Here, we describe a robust and reproducible methylation-sensitive polymerase cha
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Construction and Characterization of a Cell Line Deficient in Repair of Mitochondrial, but Not Nucle
Oxidative base lesions, such as 8-oxoguanine, accumulate in nuclear and mitochon
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Method: Preparation of Lymphocyte Cell Pellet for Storage
Method: Preparation of Lymphocyte Cell Pellet for StorageJune 10, 1990Rosalie Ve
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细胞培养中的污染问题小汇总
细胞培养中常见的污染情况总结如下: 常见的污染如下: 1、细菌 :细菌在普通倒置显微 镜下为黑色细沙状,根据感染 细菌的不同,可有不同的外形,培养液一般会浑浊变
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核糖体核糖核酸(ribosomal RNA,rRNA)
核糖体的主要组分,可达其重量的65%,原核生物中有3类rRNA,而真核生物核糖体含有4种rRNA。rRNA在构成核糖体结构和行使其生物合成功能上均有重要作用。
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Physical Monitoring of HO-Induced Homologous Recombination
The repair of chromosomal double-strand breaks (DSBs) in Saccharomyces cerevisia
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看家基因(house-keeping gene)
维持细胞最低限度功能所不可少的基因, 如编码组蛋白基因、编码核糖体蛋白基因、线粒体蛋白基因、糖酵解酶的基因等。这类基因在所有类型的细胞中都进行表达,因为这些基因
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Sperm DNA Fragmentation Analysis Using the TUNEL Assay
Terminal deoxynucleotidyl transferase-mediated deoxyuridine triphosphate-nick en
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Quantitation of Total 11-Nor-9-Carboxy-Delta 9-Tetrahydrocannabinol in Urine and Blood Using Gas Chr
Marijuana, which is made from crushing the leaves, flowers, and sometimes the st
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Flow Cytometry Analysis of Transcription Factors in T Lymphocytes
Detection of transcription factors in immune cell populations, particularly in s
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