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Quantitative Real-Time PCR Expression Analysis of Peripheral Blood Mononuclear Cells in Pancreatic C
The ability of peripheral blood mononuclear cells (PBMCs) to act as a surrogate
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High-Sensitivity Cytotoxicity Assays for Nonadherent Cells
High-sensitivity cytotoxicity assays refer to assays that can detect high levels
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Mitogen-Activated Protein Kinase Signaling in Drug-Resistant Neuroblastoma Cells
Widespread inherent or acquired resistance to cytotoxic drugs is a major limitat
2025-03-19 35 -
Yeast Two-Hybrid Screening as a Means of Deciphering Tumor Suppressor Pathways
With the identification and functional characterization of various tumor suppres
2025-03-19 35 -
Adoptive Immunotherapy
Murine models of pulmonary tumors have long provided investigators a reliable an
2025-03-19 37 -
Collagen Gel Droplet Culture Method to Examine In Vitro Chemosensitivity
For effective cancer chemotherapy, chemosensitivity testing of anticancer drugs
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Green Fluorescent Protein Retroviral Vector: Generation of High-Titer Producer Cells and Virus Super
Genes-encoding marker proteins, which are easily assayable, are useful to monito
2025-03-19 45 -
Utilizing Saccharomyces Cerevisiae to Identify Aneuploidy and Cancer Susceptibility Genes
Cancers are commonly characterized as having an abnormal number of chromosomes,
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Isolation and Culture of Melanoma and Naevus Cells and Cell Lines
Recently developed methods are described for the culture of cells from primary h
2025-03-19 42 -
DNA Methylation in Pancreatic Cancer: Protocols for the Isolation of DNA and Bisulfite Modification
Pancreatic ductal adenocarcinoma (PDAC) is an aggressive tumor and still remains
2025-03-19 46 -
Analysis of Genome-Wide DNA Methylation Profiles by BeadChip Technology
This chapter covers the genome-wide DNA methylation analysis using microarray pl
2025-03-19 38 -
Panhandle PCR Approaches to Cloning MLL Genomic Breakpoint Junctions and Fusion Transcript Sequences
Translocations and other rearrangements of the MLL gene at chromosome band 11q23
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DNA Methylation in Promoter Region as Biomarkers in Prostate Cancer
The prostate gland is the most common site of cancer and the second leading caus
2025-03-19 46 -
Digital Candidate Gene Approach (DigiCGA) for Identification of Cancer Genes
The candidate gene approach is one of the most commonly used methods for identif
2025-03-19 46 -
Microdissection and Extraction of DNA From Archival Tissue
Microdissection of cells with distinct histological features from tissue section
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