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In Vitro Chemiluminescence Assay to Measure Excision Repair in Cell Extracts
Nucleotide excision repair (NER) activity can be directly measured in whole-cell
2025-02-27 36 -
Culture Systems: Embryo Density
Embryo density is defined as the embryo-to-volume ratio achieved during in vitro
2025-02-27 39 -
Recommended working medium, trypsin volume and cell inoculation
Recommended working medium, trypsin volume and cell inoculation densityAuthor: L
2025-02-27 35 -
DNA TRANSFECTION OF EUKARYOTIC CELLS USING CALCIUM PHOS
Stock Solutions1. 2M Cacl2 Merck. Filter (to remove particulates), autoclave and
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Mapping Origins of DNA Replication in Eukaryotes
Methods are described here to map an origin of replication in eukaryotes. Replic
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HOW TO USE THE COULTER COUNTER TO COUNT CELLS
1) Turn on the counter by pulling out the on/off button. You need to do this at
2025-02-27 30 -
ES and TS cell freezing/thawing
Needed:ES cell freezing medium (2x)2x ES cell freezing medium should be made up
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血吸虫型肝硬化模型制备
所选动物：大耳白兔，体重1－3KG。 复制方法：采用腹部敷贴法感染采自钉螺的血吸虫尾蚴。兔腹部剪毛，约1CM2，滴以含尾蚴液，使每只兔感染180－200只尾蚴，
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In Vitro Fertilization and Embryo Transfer in Felids
The genome of the cat is highly conserved. In fact, of the nonprimate mammalian
2025-02-27 24 -
神经干细胞(neural stem cell，NSCs)
主要有两类：神经嵴干细胞(neuralcreststemcell，NC-SC)和中枢神经干细胞(CNS-SC)。NCSC为外周神经干细胞(PNS-SC)，既可发
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大规模培养技术应用简介
相关专题 通过大规模体外培养技术培养哺乳类动物细胞是生产生物制品的有效方法。20世界60-70年代，就已创立了可用于大规模培养动物细胞的微载体培养系统和中空
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Retrovirus-Mediated RNA Interference
RNA interference (RNAi) has recently emerged as a reliable tool for studying the
2025-02-27 36 -
Protocols for Investigating microRNA Functions in Human Neural Progenitor Cells
Human embryonic stem cells and induced pluripotent stem cells offer great hope f
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避免牛血清沉淀的小技巧
相关专题 细胞培养基的配制 细胞培养专题小技巧之避免牛血清沉淀:各位童鞋在细胞培养 过程中，应该遇到过使用的牛血清产生沉淀的问题，溶解后有沉淀，往往还怕是染菌，
2025-02-27 27 -
The Scientist：活细胞病毒观察指南
相关专题对于大多数生物学过程来说，所见即所得。由于病毒在感染性疾病中的作用，许多生物学家希望能够实时观察病毒的活动。要观察病毒感染细胞和病毒装配的过程，荧光成像
2025-02-27 34
