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Cell-Based Arrays for the Identification of Interacting Polypeptide Domains or Epitopes
The specific regions on proteins which are responsible for protein–protein inter
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Cell-Based Reporter Assay to Analyze Activation of Nod1 and Nod2
Nod1 and Nod2 are pattern recognition receptors of the mammalian innate immune s
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Cell-Free Assays: The Reductionist Approach to the Study of NADPH Oxidase Assembly, or All You Wante
The superoxide (O2 •− )-generating enzyme complex of phagocytes, known as the NA
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Cell-Free Bead-Based Detection of Total and Phosphorylated Proteins in Plasma and Cell Lysates: Dete
Frequently direct measurement of proteins or their phosphorylation in intact cel
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Cell-Free Selection of Domain Antibodies by In Vitro Compartmentalization
Efficient identification of antibodies, or any fragments thereof, displaying des
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CELLISA: Reporter Cell-Based Immunization and Screening of Hybridomas Specific for Cell Surface Anti
Monoclonal antibodies (mAbs) specific for cell surface antigens are an invaluabl
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Cellular Expression of A20 and ABIN-3 in Response to Toll-Like Receptor-4 Stimulation
Although Toll-like receptor (TLR)-induced expression of several proinflammatory
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Cellular Human and Viral DNA Detection by Nonisotopic In Situ Hybridization
In situ hybridization may be defined as the detection of nucleic acids in situ i
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Cellular siRNA Delivery Using TatU1A and Photo-Induced RNA Interference
RNA interference (RNAi)-mediated silencing of specific genes represents a powerf
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Cellular ELISA Protocol
Formalin Fixed Cell Plates1. Trypsinize confluent flasks2. Pool and count cells3
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CFU-A Assay for Measurement of the Antiproliferative Effects of Chemokines on Murine Early Hemopoiet
One of the more intriguing features of the hemopoietic system is the ability of
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Challenges and Opportunities in Fractionation of Recombinant Human Mannan-Binding Lectin
Mannan-binding lectin is an oligomeric protein of the innate immune system. Both
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Chaperonins in Phage Display of Antibody Fragments
The display of antibody fragments on the surface of filamentous bacteriophages (
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CHAPS Insoluble Floating Fraction Preparation
CHIFF preparationDevelop cells for at least 3 hrs to minimize protease activity.
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Characterisation of Viral Proteins that Inhibit Toll-Like Receptor Signal Transduction
Toll-like receptor (TLR) signalling involves five TIR adapter proteins, which co
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