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Preparing chemically competent cells
MaterialsPlate of cells to be made competentTSS bufferLB mediaIceGlassware & Equ
2025-02-26 41 -
Live Imaging of Drosophila Embryos: Quantifying Protein Numbers and Dynamics at Subcellular Location
Live imaging is critical for understanding the structure and activities of prote
2025-02-26 32 -
Identification of Target Genes of a Yeast Transcriptional Repressor
Xbp1 is a transcriptional repressor with homology to the DNA binding domains of
2025-02-26 38 -
Analysis of Skeletal Muscle Development in Drosophila
The Drosophila system has been invaluable in providing important insights into m
2025-02-26 22 -
Studies of Adult Neural Stem Cell Migration
Adult neural progenitors originating is the subventricular zone (SVZ) proceed to
2025-02-26 26 -
Beads on the Run: Beads as Alternative Tools for Chemotaxis Assays
Cell migration is required for a wide variety of processes from bacteria seeking
2025-02-26 30 -
胸腹水染色体标本制备
一、原理 某些肿瘤细胞可脱落入胸腹水中，并继续生长、增值。因此，由胸腹水细胞可制备较好的染色体标本。胸腹水细胞染色体分析对恶性肿瘤具有辅助诊断的价值。如
2025-02-26 29 -
Approaches to Cloning of Pain-Related Ion Channel Genes
Molecular pain research is a relatively new and rapidly expanding field that rep
2025-02-26 24 -
ES Cell-Mediated Conditional Transgenesis
The use of mouse embryonic stem (ES) cells to alter the mouse genome has become
2025-02-26 34 -
细胞培养的微生物污染排除
一、抗生素除菌法：用BM－1（截耳素衍生物）和BM－2（四环素衍生物）抑制支原体1．抗生素制备：均可用PBS配成250浓缩液－20℃备用，使用浓度参考《组织培养
2025-02-26 137 -
Imaging Lipid Bodies Within Leukocytes with Different Light Microscopy Techniques
Lipid bodies, also known as lipid droplets, are present in most eukaryotic cells
2025-02-26 35 -
小白鼠染色体的制备和观察
相关专题 一、实验目的 通过实验，掌握空气干燥法制片技术。 二、实验原理 在骨髓细胞中，有丝分裂的指数是相当高的，因此可以直接得到中期细胞而不必像血淋巴细胞
2025-02-26 37 -
交换
在减数分裂Ⅰ的粗线期发生的同源染色体的非姊妹染色单体间的局部交换，以及连锁的基因重组。 1964年 R． Holliday从分子水平对交换做出解释，认为二价体染
2025-02-26 35 -
Introduction of Genes Into Primary Murine Splenic B Cells Using Retrovirus Vectors
Primary murine splenic B cells can be cultured ex vivo and, following treatment
2025-02-26 25 -
RNA Extraction From Drosophila Heads
In Drosophila , input, pacemaker, and output genes are expressed circadianly. mR
2025-02-26 31
