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  • Development of Immortalized Hepatocyte-Like Cells from hMSCs

    Development of Immortalized Hepatocyte-Like Cells from hMSCs

    Clones of hepatocyte-like cells were reproducibly generated from human mesenchym

    2025-02-25 32
  • Adipose-Derived Stromal Cells: Cytokine Expression and Immune Cell Contaminants

    Adipose-Derived Stromal Cells: Cytokine Expression and Immune Cell Contaminants

    The present method describes an immunoselection/depletion approach to isolate th

    2025-02-25 22
  • Primary Culture and Differentiation of Human Adipocyte Precursor Cells

    Primary Culture and Differentiation of Human Adipocyte Precursor Cells

    Adipose or fat tissue is of mesenchymal origin and is comprised of adipocytes, a

    2025-02-25 28
  • DNA Damage Quantitation by Alkaline Gel Electrophoresis

    DNA Damage Quantitation by Alkaline Gel Electrophoresis

    Physical and chemical agents in the environment, those used in clinical applicat

    2025-02-25 30
  • Patch-Clamping Drosophila Sensory Neurons

    Patch-Clamping Drosophila Sensory Neurons

    Electrophysiological studies provide essential clues about the regulation and ph

    2025-02-25 27
  • In Vivo Quantitative Proteomics: The SILAC Mouse

    In Vivo Quantitative Proteomics: The SILAC Mouse

    Mass spectrometry-based proteomics is a field that has been quickly developing,

    2025-02-25 24
  • A Protocol for Heterologous Expression and Functional Assay for Mouse Pheromone Receptors

    A Protocol for Heterologous Expression and Functional Assay for Mouse Pheromone Receptors

    Innate social behaviors like intermale aggression, fear, and mating rituals are

    2025-02-25 26
  • Nonclinical and Clinical Experiences with CPP-Based Self-Assembling Peptide Systems in Topical Drug

    Nonclinical and Clinical Experiences with CPP-Based Self-Assembling Peptide Systems in Topical Drug

    Considerations in rational designs of CPP-based transcutaneous delivery systems

    2025-02-25 24
  • Generation and Characterization of MacGreen Mice, the Cfs1r-EGFP Transgenic Mice

    Generation and Characterization of MacGreen Mice, the Cfs1r-EGFP Transgenic Mice

    Macrophage colony-stimulating factor (CSF-1) regulates the differentiation, prol

    2025-02-25 30
  • Assisted Reproductive Technology in Nonhuman Primates

    Assisted Reproductive Technology in Nonhuman Primates

    Nonhuman primates (NHP) are the closest animal species to humans and have been w

    2025-02-25 29
  • Imaging of Intracellular Hydrogen Peroxide Production with Hyper Upon Stimulation of Hela Cells with

    Imaging of Intracellular Hydrogen Peroxide Production with Hyper Upon Stimulation of Hela Cells with

    Reactive oxygen species (ROS) regulate both normal cell functions by activating

    2025-02-25 30
  • Methods for the Purification of HQ-Tagged Proteins

    Methods for the Purification of HQ-Tagged Proteins

    The HQ (H = histidine, Q = glutamine) tag is a small fusion tag that can be isol

    2025-02-25 19
  • Culturing HEK 293 Cells

    Culturing HEK 293 Cells

    ReagentsMedium: 500 ml Dulbecco’s Modified Eagle Medium (Gibco #41966-029) 55 ml

    2025-02-25 28
  • 细胞生物基本方法:复苏

    细胞生物基本方法:复苏

    复苏方法1. 从液氮罐中取出安剖;有时因安剖未封严,浸入了液氮,取出后因安剖内液氮迅速气化而发生爆炸,因此应带有防护眼睛和手套。2. 迅速放入盛有 36 ℃~

    2025-02-25 28
  • Xenopus Oocyte Wound Healing as a Model System for Analysis of Microtubule-Actin Interactions

    Xenopus Oocyte Wound Healing as a Model System for Analysis of Microtubule-Actin Interactions

    Microtubule-actin interactions are fundamental to many cellular processes such a

    2025-02-25 20