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Development of Immortalized Hepatocyte-Like Cells from hMSCs
Clones of hepatocyte-like cells were reproducibly generated from human mesenchym
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Adipose-Derived Stromal Cells: Cytokine Expression and Immune Cell Contaminants
The present method describes an immunoselection/depletion approach to isolate th
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Primary Culture and Differentiation of Human Adipocyte Precursor Cells
Adipose or fat tissue is of mesenchymal origin and is comprised of adipocytes, a
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DNA Damage Quantitation by Alkaline Gel Electrophoresis
Physical and chemical agents in the environment, those used in clinical applicat
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Patch-Clamping Drosophila Sensory Neurons
Electrophysiological studies provide essential clues about the regulation and ph
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In Vivo Quantitative Proteomics: The SILAC Mouse
Mass spectrometry-based proteomics is a field that has been quickly developing,
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A Protocol for Heterologous Expression and Functional Assay for Mouse Pheromone Receptors
Innate social behaviors like intermale aggression, fear, and mating rituals are
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Nonclinical and Clinical Experiences with CPP-Based Self-Assembling Peptide Systems in Topical Drug
Considerations in rational designs of CPP-based transcutaneous delivery systems
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Generation and Characterization of MacGreen Mice, the Cfs1r-EGFP Transgenic Mice
Macrophage colony-stimulating factor (CSF-1) regulates the differentiation, prol
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Assisted Reproductive Technology in Nonhuman Primates
Nonhuman primates (NHP) are the closest animal species to humans and have been w
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Imaging of Intracellular Hydrogen Peroxide Production with Hyper Upon Stimulation of Hela Cells with
Reactive oxygen species (ROS) regulate both normal cell functions by activating
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Methods for the Purification of HQ-Tagged Proteins
The HQ (H = histidine, Q = glutamine) tag is a small fusion tag that can be isol
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Culturing HEK 293 Cells
ReagentsMedium: 500 ml Dulbecco’s Modified Eagle Medium (Gibco #41966-029) 55 ml
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细胞生物基本方法:复苏
复苏方法1. 从液氮罐中取出安剖;有时因安剖未封严,浸入了液氮,取出后因安剖内液氮迅速气化而发生爆炸,因此应带有防护眼睛和手套。2. 迅速放入盛有 36 ℃~
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Xenopus Oocyte Wound Healing as a Model System for Analysis of Microtubule-Actin Interactions
Microtubule-actin interactions are fundamental to many cellular processes such a
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