-
Preparation of Synchronized Human Cell Extracts to Study Ubiquitination and Degradation
Ubiquitination and protein degradation regulate cell cycle progression in all eu
2025-02-24 18 -
Purification of Murine Monoclonal Antibodies
Hybridoma technology has made possible the production of highly specific, homoge
2025-02-24 17 -
Characterization of Enzyme Activity, Protein Content, and Thiol Groups in Immobilized Enzymes
Immobilization is designed to restrict the freedom of movement of an enzyme and,
2025-02-24 22 -
Oocyte Isolation and Enucleation
Xenopus laevis oocytes are popular cells in experimental biology. Fully grown oo
2025-02-24 21 -
Studying Cytoskeletal Dynamics in Living Cells Using Green Fluorescent Protein
The cytoskeleton is a dynamic structure comprised of at least three distinct cel
2025-02-24 20 -
Determination of Gene Expression Patterns by Whole-Mount In Situ Hybridization
Whole-mount in situ hybridization (WISH) is a reliable and specific method to st
2025-02-24 24 -
Detection of Apoptosis in the Central Nervous System
Apoptosis occurs in the nervous system during normal development, but can also b
2025-02-24 22 -
Serum-Resistant Lipoplexes in the Presence of Asialofetuin
Vectors proposed for gene delivery generally fall into two categories: viral and
2025-02-24 20 -
Isolation and Characterization of Primitive Hematopoietic Cells Based on Their Position in the Cell
Hematopoietic progenitor and stem cells are believed to lie dormant within the a
2025-02-24 18 -
Cytokine Release in Tissue-Engineered Epidermal Equivalents After Prolonged Mechanical Loading
Prolonged mechanical loading of soft tissues may result in degeneration of these
2025-02-24 20 -
Epithelial-Mesenchymal Interactions
Cell-cell and tissue-tissue interactions initiate and regulate genomic expressio
2025-02-24 18 -
自由基(free radical)
自由基是指那些在原子核外层轨道上具有不成对电子的分子或原子基团。所谓未成对电子,就是指那些在原子或分子轨道中未与其它电子配对而独占一个轨道的电子。如AB两个原子
2025-02-24 23 -
DNA Microarray for Molecular Epidemiology of Salmonella
Salmonellosis is a common infection estimated to affect 3 billion people and to
2025-02-24 18 -
Nuclear Reprogramming of Somatic Nucleus Hybridized With Embryonic Stem Cells by Electrofusion
Cell fusion is a powerful tool for understanding the molecular mechanisms of epi
2025-02-24 17 -
Ligase Chain Reaction for Site-Directed In Vitro Mutagenesis
Before the advent of polymerase chain reaction (PCR) technology, many methods fo
2025-02-24 22
