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兔成骨细胞与骨髓基质干细胞混合培养的实验研究
对骨髓基质干细胞进行改造,使其在保持快速生长的同时向成骨细胞方向分化是目前国内外研究的热点。实验发现,成熟的成骨细胞可以通过细胞间的直接接触作用促进骨髓基质干细
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Isolation, Culture, and Differentiation of Progenitor Cells from the Central Nervous System
Isolation, Culture, and Differentiation of Progenitor Cells from the Central Ner
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Analysis of Signal Transduction: Formation of cAMP, cGMP, and Ins(1,4,5)P3 In Vivo and In Vitro
This chapter describes biochemical assays with which to analyze signal transduct
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Cytoplasmic mRNA Polyadenylation and Translation Assays
Vertebrate development is directed by maternally inherited messenger RNAs that a
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重链结合蛋白(heavy-chain binding protein, Bip)
Bip是重链结合蛋白的简称,因为它能够同IgG抗体的重链结合而得名。Bip是一类分子伴侣,属于Hsp70家族,在内质网中有两个作用。第一,Bip同进入内质网的未
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Double-Label Immunoelectron Microscopy for Studying the Colocalization of Proteins in Cultured Cells
Multiple label immunoelectron microscopy localizes and detects multiple antigens
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Immunohistochemical Staining of Cyclooxygenases with Monoclonal Antibodies
Immunohistochemistry is an important tool that is often used for the diagnosis o
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Preparation of cytospin from single cell suspension.
Cell number, speed and trivial details affect cytospin .Basic protocol:Prepare a
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细胞凋亡实验
相关专题 一、实验目的: 1、掌屋凋亡细胞的形态特征 2、学会用荧光探针 对细胞进行双标记来检测正常活细胞、凋亡细胞和坏死细胞的方法 二、实验原理 细胞死亡
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Noble Agar Assay
Description Cancer cells do not show anchorage and contact inhibition of growth.
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骨髓间充质干细胞的主要表面标志
1 骨髓间充质干细胞的发现和来源骨髓组织 中有多种细胞成分, 除基质细胞等已经分化的细胞外,还含有两类多潜能干细胞:造血干细胞和间充质干细胞。 1987 年F
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Staining Methods for cell
death Z. Xia 10/2/95 The simplest way: trypan blue. Dead cells stain blue Non-fi
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实验:用淋巴细胞分离液分离单个核细胞
相关专题 1)分离外周血中的单个核细胞(PBMC) 1.抽取正常人静脉血加到肝素抗凝管中,加等量含5~10IU/ml肝素的无血清缓冲液悬浮细胞。将细胞悬液小心
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细胞自发转化spontenous transformation
1.什么是细胞转化?何时会发生细胞转化? 细胞转化是细胞发生遗传性状改变的一种变化,它的基础是DNA或基因的突变。从属性上说,基因突变导致生物体发生的转化,有利
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支原体(mycoplasma)
又称霉形体,是最简单的原核细胞,支原体的大小介于细菌与病毒之间,直径为0.1~0.3 um, 约为细菌的十分之一, 能够通过滤菌器。支原体形态多变,有圆形、丝状
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