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Transfection of Osteoclasts and Osteoclast Precursors
Osteoclasts and their precursors have traditionally been considered difficult ce
2025-02-22 19 -
Isolation and Culture of Human Renal Cortical Cells with Characteristics of Proximal Tubules
The kidney is an extremely heterogeneous organ from a morphological and a functi
2025-02-22 15 -
Method: Reactivating Cell Lines and Cell Growth for DNA Preparation
urpose: Cell lines are reactivated and grown to a count of 1 x 108 cells. The c
2025-02-22 19 -
Site-Directed Mutagenesis in the Study of Membrane Transporters
One of the major goals in membrane transporter research is to understand how tra
2025-02-22 18 -
Analysis of Proteins from Marine Molluscs
Application of the two-dimensional gel electrophoresis (2DE) protocols which wer
2025-02-22 19 -
The Effect of Lipid Adjustment on the Analysis of Environmental Contaminants and the Outcome of Huma
Past literature on exposure to lipophilic agents such as organochlorines (OCs) i
2025-02-22 21 -
The Use of Calorie Restriction Mimetics to Study Aging
Calorie restriction (CR) has a variety of effects on extending lifespan and dela
2025-02-22 25 -
Preparation of Astrocyte, Oligodendrocyte, and Microglia Cultures from Primary Rat Cerebral Cultures
The study of glial cell development and function has been considerably enhanced
2025-02-22 17 -
MMP-9 and TIMP-1 Assays in Keratinocyte Cultures
Matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TI
2025-02-22 25 -
Immunoelectron Microscopy of Cryofixed and Freeze-Substituted Plant Tissues
Cryofixation and freeze-substitution techniques preserve plant ultrastructure mu
2025-02-22 18 -
PKA系统(protein kinase A system, PKA)
是G蛋白偶联系统的一种信号转导途径。信号分子作用于膜受体后,通过G蛋白激活腺苷酸环化酶, 产生第二信使cAMP后,激活蛋白激酶A进行信号的放大。故将此途径称为P
2025-02-22 35 -
Preparation and Use of Liposomes for the Study of Sphingolipid Segregation in Membrane Model Systems
Several investigations, carried out in either artificial or cellular models and
2025-02-22 19 -
Measurement of Caspase Activity in Cells Undergoing Apoptosis
Cysteine proteases of the caspase family play key roles in the execution of apop
2025-02-22 19 -
Cell Fusion Assays for Yeast Mating Pairs
Yeast mating provides an accessible genetic system for the discovery of fundamen
2025-02-22 21 -
Standardized Isolation of Human Mesenchymal Stromal Cells with Red Blood Cell Lysis
Human mesenchymal stromal cells (MSC) raise high hopes for tissue engineering an
2025-02-22 24