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  • Transfection of Osteoclasts and Osteoclast Precursors

    Transfection of Osteoclasts and Osteoclast Precursors

    Osteoclasts and their precursors have traditionally been considered difficult ce

    2025-02-22 19
  • Isolation and Culture of Human Renal Cortical Cells with Characteristics of Proximal Tubules

    Isolation and Culture of Human Renal Cortical Cells with Characteristics of Proximal Tubules

    The kidney is an extremely heterogeneous organ from a morphological and a functi

    2025-02-22 15
  • Method: Reactivating Cell Lines and Cell Growth for DNA Preparation

    Method: Reactivating Cell Lines and Cell Growth for DNA Preparation

    urpose: Cell lines are reactivated and grown to a count of 1 x 108 cells. The c

    2025-02-22 19
  • Site-Directed Mutagenesis in the Study of Membrane Transporters

    Site-Directed Mutagenesis in the Study of Membrane Transporters

    One of the major goals in membrane transporter research is to understand how tra

    2025-02-22 18
  • Analysis of Proteins from Marine Molluscs

    Analysis of Proteins from Marine Molluscs

    Application of the two-dimensional gel electrophoresis (2DE) protocols which wer

    2025-02-22 19
  • The Effect of Lipid Adjustment on the Analysis of Environmental Contaminants and the Outcome of Huma

    The Effect of Lipid Adjustment on the Analysis of Environmental Contaminants and the Outcome of Huma

    Past literature on exposure to lipophilic agents such as organochlorines (OCs) i

    2025-02-22 21
  • The Use of Calorie Restriction Mimetics to Study Aging

    The Use of Calorie Restriction Mimetics to Study Aging

    Calorie restriction (CR) has a variety of effects on extending lifespan and dela

    2025-02-22 25
  • Preparation of Astrocyte, Oligodendrocyte, and Microglia Cultures from Primary Rat Cerebral Cultures

    Preparation of Astrocyte, Oligodendrocyte, and Microglia Cultures from Primary Rat Cerebral Cultures

    The study of glial cell development and function has been considerably enhanced

    2025-02-22 17
  • MMP-9 and TIMP-1 Assays in Keratinocyte Cultures

    MMP-9 and TIMP-1 Assays in Keratinocyte Cultures

    Matrix metalloproteinases (MMPs) and tissue inhibitors of metalloproteinases (TI

    2025-02-22 25
  • Immunoelectron Microscopy of Cryofixed and Freeze-Substituted Plant Tissues

    Immunoelectron Microscopy of Cryofixed and Freeze-Substituted Plant Tissues

    Cryofixation and freeze-substitution techniques preserve plant ultrastructure mu

    2025-02-22 18
  • PKA系统(protein kinase A system, PKA)

    PKA系统(protein kinase A system, PKA)

    是G蛋白偶联系统的一种信号转导途径。信号分子作用于膜受体后,通过G蛋白激活腺苷酸环化酶, 产生第二信使cAMP后,激活蛋白激酶A进行信号的放大。故将此途径称为P

    2025-02-22 35
  • Preparation and Use of Liposomes for the Study of Sphingolipid Segregation in Membrane Model Systems

    Preparation and Use of Liposomes for the Study of Sphingolipid Segregation in Membrane Model Systems

    Several investigations, carried out in either artificial or cellular models and

    2025-02-22 19
  • Measurement of Caspase Activity in Cells Undergoing Apoptosis

    Measurement of Caspase Activity in Cells Undergoing Apoptosis

    Cysteine proteases of the caspase family play key roles in the execution of apop

    2025-02-22 19
  • Cell Fusion Assays for Yeast Mating Pairs

    Cell Fusion Assays for Yeast Mating Pairs

    Yeast mating provides an accessible genetic system for the discovery of fundamen

    2025-02-22 21
  • Standardized Isolation of Human Mesenchymal Stromal Cells with Red Blood Cell Lysis

    Standardized Isolation of Human Mesenchymal Stromal Cells with Red Blood Cell Lysis

    Human mesenchymal stromal cells (MSC) raise high hopes for tissue engineering an

    2025-02-22 24