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Measurement of UV Radiation-Induced DNA Damage Using Specific Antibodies
Measurement of DNA damage can be difficult if the levels of damage are small. Fo
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CD8+, CD8, and Plasmacytoid Dendritic Cell Generation In Vitro Using flt3 Ligand
The generation of dendritic cells (DCs) from monocytes and early progenitors in
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Bone Marrow-Derived Mesenchymal Stem Cells: Isolation, Expansion, Characterization, Viral Transducti
Mesenchymal stem cells (MSCs) are defined as self-renewing and multipotent cells
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MTT方法的使用步骤
1:接种细胞:用含10%胎小牛血清得培养液配成单个细胞悬液,以每孔 1000-10000个细胞接种到96孔板,每孔体积200ul. 2:培养细胞:同一般培养条件
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糖原的显示―PAS反应(periodic acid schiff’s reaction)
目的要求 通过PAS反应,了解糖原显示的基本原理、方法以及糖原在细胞中的分布。 基本原理 糖原由D-葡萄糖的分支或直链组成,在肝和肌肉最丰富。过碘酸是一种强氧化
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Human Embryo Culture and Assessment for the Derivation of Embryonic Stem Cells (ESC)
The culture and critical assessment of early human embryos during the first week
2025-02-21 25 -
Using 2D Crystals to Analyze the Structure of Membrane Proteins
Electron crystallography is a powerful technique for studying the structure and
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细胞培养基中常用维生素及其作用
相关专题 细胞培养基的配制维持细胞生长的生物活性物质,在细胞代谢中起调节及控制作用。在细胞培养 中,尽管血清是维生素重要来源, 但是许多培养基中添加了各种维生素
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Cloning of Potassium Channel Splice Variants from Tissues and Cells
Potassium channels display considerable functional diversity. Alternative pre-mR
2025-02-21 28 -
Immunohistochemistry of Xenopus Embryos
Immunohistochemistry is a very powerful technique for determining both the tissu
2025-02-21 22 -
Isolation of Hair Follicle Bulge Stem Cells from YFP-Expressing Reporter Mice
In this article we provide a method to isolate hair follicle stem cells that hav
2025-02-21 25 -
Nucleotide Excision Repair in Saccharomyces cerevisiae Whole-Cell Extracts
Nucleotide excision repair (NER) is a particularly versatile pathway of DNA repa
2025-02-21 18 -
Functional Assays for Hematopoietic Stem Cell Self-Renewal
Stem cells are defined by the ability to self-renew. Specific functional assays
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Biomarkers of Cell Senescence Assessed by Imaging Cytometry
The characteristic features of senescent cells such as their “flattened” appeara
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PMEF FEEDER LAYER CONCENTRATION
Area/WellTotal AreaTotal CellsTotal VolConc/ml Flask cm2/cm25x104/cm2 96 Well0.
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