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Reconstitution of G Protein-Coupled Receptors into a Model Bilayer System: Reconstituted High-Densit
Reconstituted high-density lipoprotein particles (rHDL) are powerful platforms u
2025-02-20 21 -
Genetic Manipulation of Keratinocyte Stem Cells with Lentiviral Vectors
The epidermis of the skin and its appendages, such as the hair follicles, are fo
2025-02-20 21 -
Generation of Murine Stromal Cell Lines: Models for the Microenvironment of the Embryonic Mouse Aort
We describe a method to derive cell lines and clones from cells of the murine mi
2025-02-20 24 -
Studying Nuclear Reprogramming with Cell Hybrids
Every cell in a mammal, other than odd exceptions (e.g. mature erythrocytes or l
2025-02-20 25 -
The Identification of Subcellular Fractions from Mammalian Cells
The chapters in the two volumes of Biomembrane Protocols are broadly devoted to
2025-02-20 24 -
Synchronization of Cell Populations in G1/S and G2/M Phases of the Cell Cycle
The method described in the following chapter utilizes a double thymidine block
2025-02-20 35 -
Methods for Studying Checkpoint Kinases Chk1
Attempts to passage through mitosis with unrepaired DNA damage or incompletely r
2025-02-20 25 -
Prespliceosome and Spliceosome Isolation and Analysis
Spliceosomes are multicomponent enzymes that remove introns from premessenger RN
2025-02-20 27 -
Long-Term B-Lymphoid Cultures from Murine Bone Marrow Establishment and Cloning by Using Stromal Cel
Nearly all hematopoietic cells in mammals derive from precursors that undergo mu
2025-02-20 27 -
Quantification of Fungal Infection of Leaves with Digital Images and Scion Image Software
Digital image analysis has been used to distinguish and quantify leaf color chan
2025-02-20 20 -
GeneCalling: Transcript Profiling Coupled to a Gene Database Query
We describe here the GeneCalling� method for the discovery of differentially exp
2025-02-20 20 -
Deep Sequencing of Small Chromatin-Associated RNA: Bioinformatic Analysis
Chromatin-associated RNA (caRNA) is a newly identified class of RNA stably linke
2025-02-20 20 -
Recognition Imaging of Chromatin and Chromatin-Remodeling Complexes in the Atomic Force Microscope
Atomic force microscopy (AFM) can directly visualize single molecules in solutio
2025-02-20 22 -
Posttranscriptional Regulation of Gene Expression in Epithelial Cells by Polyamines
In addition to regulating gene transcription, polyamines also potently modulate
2025-02-20 22 -
The Calvarial Injection Assay
This chapter describes the calvarial injection method, whereby the effect of a s
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