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Amylose Affinity Chromatography of Maltose-Binding Protein: Purification by both Native and Novel Ma
Maltose-binding protein (MBP) is a carrier protein for high level recombinant pr
2025-02-19 36 -
Isolation, Propagation, and Characterization of Human Umbilical Cord Perivascular Cells (HUCPVCs)
Current sources of mesenchymal cells, including bone marrow, fat and muscle, all
2025-02-19 28 -
Murine Bone Marrow-Derived Macrophages
The molecular phagocytic lineage comprises, in order of increasing maturity, the
2025-02-19 24 -
纺锤体（spindle）
大量微管纵向排列组成的中间宽两极小的细胞器，形状象纺锤，因而得名。因为纺锤体和染色体运动密切相关，所以纺锤体是一个重要的有丝分裂装置。纺锤体由极间丝（又叫连续
2025-02-19 57 -
Sos蛋白(Sos protein)
Sos蛋白是编码鸟苷释放蛋白的基因sos的产物（sos是son of sevenless 的缩写）。Sos蛋白在Ras信号转导途径中的作用是促进Ras释放GDP
2025-02-19 26 -
Melanosome Motility in Fish Retinal Pigment Epithelial Cells
Several model systems have been developed to investigate intracellular organelle
2025-02-19 30 -
Visualization of Induced RNA in Single Bacterial Cells
Visualization of RNA in live cells is a challenging task due to the transient ch
2025-02-19 31 -
Isolation and Short-Term Culture of Human Epidermal Langerhans Cells
The human epidermis consists of a heterogeneous population of cells including ke
2025-02-19 32 -
Visualization of Spindle Behavior Using Confocal Microscopy
Numerous comparisons have shown that confocal imaging of fluorescently labeled s
2025-02-19 27 -
Yeast Protoplast Fusion
A major limitation to the results that could be achieved using traditional metho
2025-02-19 26 -
Immobilization of Enzymes and Proteins on Red Blood Cells
Immobilization of proteins on intact red blood cells (RBCs) can be valuable for
2025-02-19 31 -
The Measurement of Protein Degradation in Response to Oxidative Stress
Molecular oxygen is an excellent acceptor of electrons and is therefore employed
2025-02-19 29 -
Cell Viability Assays: Introduction
The measurement of cell viability plays a fundamental role in all forms of cell
2025-02-19 22 -
In Situ Hybridization with Nonradioactive Probes
In situ hybridization takes advantage of the reaction between two complementary
2025-02-19 26 -
Analysis of Fungal Gene Expression by Real Time Quantitative PCR
The Real-Time quantitative PCR (qPCR) method has become central for the quantifi
2025-02-19 21
