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MSCs的分离
1.从你叙述的情况我分析是percoll的问题，只有分离效果不好时才会出现中间层红细胞的残留。当然你可以全骨髓培养一下，以证明细胞活力的好坏 2.我用的perc
2025-03-07 64 -
染色体骨架(scaffold)
在染色体包装时, 为染色质提供锚定位点的非组蛋白。为了证明染色体骨架的存在, 分离有丝分裂前的染色体, 接着用试剂溶解组蛋白和大多数主要的非组蛋白, 然后在电子
2025-03-07 56 -
原位末端转移酶标记技术检测凋亡
（一）原理 凋亡细胞是由于内源性核酸内切酶的激活后，将DNA 切割成许多双链DNA 片段以及高分子量DNA 单链断裂点（缺口），暴露出大量3- 羟基末端，如用末
2025-03-07 73 -
Cell Quantification
Aim For the majority of manipulations using cell cultures, such as transfections
2025-03-07 56 -
细胞培养实验中常用器材和试剂的配制
相关专题之前讲述了细胞培养 的常用的两个工具即细胞培养瓶和细胞培养板，这次再来说下细胞培养常用的器材和试剂，主要讲述了细胞培养常用试剂的配制。细胞培养常用的器材
2025-03-07 69 -
凝集素(lectin)
凝集素是动物细胞和植物细胞都能够合成和分泌的、能与糖结合的蛋白质,在细胞识别和粘着反应中起重要作用,主要是促进细胞间的粘着。凝集素具有一个以上同糖结合的位点，因
2025-03-07 51 -
M6P受体蛋白(M6P receptor protein)
M6P受体蛋白是反面高尔基网络上的膜整合蛋白, 能够识别溶酶体水解酶上的M6P信号并与之结合, 从而将溶酶体的酶蛋白分选出来，然后通过出芽的方式将溶酶体的酶蛋白
2025-03-07 83 -
Gas Chromatography-Mass Spectrometry Method for the Determination of Methadone and 2-Ethylidene-1,5-
Methadone is a synthetic opioid used to relieve pain, treat opioid withdrawal, a
2025-03-07 60 -
Fluorescence Correlation Spectroscopy (FCS)-Based Characterisation of Aptamer Ligand Interaction
Fluorescence correlation spectroscopy (Bacia and Schwille (2007) Nat. Protoc. 2
2025-03-07 36 -
Post-translational Modification of p53 by Ubiquitin
Post-translational modification of p53 by ubiquitin resides in the center of a f
2025-03-07 43 -
Detection of DNA-Dependent Protein Kinase in Extracts from Human and Rodent Cells
The DNA-dependent protein kinase, DNA-PK, is required for DNA double-strand brea
2025-03-07 55 -
293-T细胞培养实验中注意事项
相关专题培养293细胞 应注意以下几个方面：1.用1640加10%胎牛血清，有的种系用DMEM .血清要求质量较高，在普通血清中细胞生长不太好。1640用双蒸水
2025-03-07 58 -
Ribonuclease Protection Analysis of Gene Expression in Xenopus
When characterizing the developmental expression of a novel gene, or when examin
2025-03-07 49 -
Observational Study Design
Much can be learned about a process by observing changes over time or by compari
2025-03-07 48 -
Cell Migration Analyses Within Fibroblast-Derived 3-D Matrices
Research in cell biology often is based in tissue culturing cells on artificial
2025-03-07 44
