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Isolation of Actin and Myosin Filaments
Isolation of Actin and Myosin FilamentsLEVEL IIIMaterials Relaxing Solution0.1 M
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Study of Cell Cycle Checkpoints Using Xenopus Cell-Free Extracts
Cell cycle checkpoints are involved in the coordinated response to DNA damage an
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In Vivo Skin-Targeted Gene Delivery by Pulsed Electric Fields
The skin is an especially attractive target for gene therapy. In particular, the
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Constitutively Active STAT5 Constructs
The transcription factor S ignal T randucer and A ctivator of T ranscription 5 (
2025-02-19 19 -
Efficient Generation of Dopamine Neurons from Human Embryonic Stem Cells
In this chapter, we introduce a co-culture protocol for human embryonic stem (hE
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Directed Differentiation of Neural-stem cells and Subtype-Specific Neurons from hESCs
We describe a chemically defined protocol for efficient differentiation of human
2025-02-19 19 -
Visualization of Sperm Accessory Structures in the Mammalian Spermatids, Spermatozoa, and Zygotes by
Mammalian fertilization is traditionally viewed as a process during which the fe
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Cell Synchronization by Inhibitors of DNA Replication Induces Replication Stress and DNA Damage Resp
Cell synchronization is often achieved by inhibition of DNA replication. The cel
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Sequencing of (dA:dT) Cloned Mixed PCR Products from Microbial Populations
Because only between 1 and 10% of bacteria present in soil and aquatic environme
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A High-Throughput Mammalian Cell-Based Transient Transfection Assay
In eukaryotic organisms gene expression is regulated through a variety of upstre
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锚定蛋白(ankyrin)
又称2.1蛋白。锚定蛋白是一种比较大的细胞内连接蛋白, 每个红细胞约含10万个锚定蛋白，相对分子质量为215,000。锚定蛋白一方面与血影蛋白相连, 另一方面与
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Stepwise Differentiation from Nave State Pluripotent Stem Cells to Functional Primordial Germ Cells
A group of pluripotent cells appearing during mammalian embryogenesis is the sou
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An In Situ Hybridization Technique to Detect Low-Abundance Slug mRNA in Adherent Cultured Cells
In this chapter, we describe a simple and relatively rapid technique for detecti
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Vertical Arrays: Microarrays of Complex Mixtures of Nucleic Acids
Vertical arrays are microarrays that have complex mixtures of nucleic acids as a
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Fusion Protocol for the Production of Mouse Hybridomas
Hybridoma technology makes it possible to produce almost unlimited amounts of mo
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