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  • Differential mRNA Display: Adaption for In Vivo Studies of Diseased Tissues

    Differential mRNA Display: Adaption for In Vivo Studies of Diseased Tissues

    This section focuses on the application of differential display in gaining insig

    2025-02-20 28
  • Production of Knockdown Rats by Lentiviral Transduction of Embryos with Short Hairpin RNA Transgenes

    Production of Knockdown Rats by Lentiviral Transduction of Embryos with Short Hairpin RNA Transgenes

    The primary method for determining the function of a gene in rodents has been to

    2025-02-20 28
  • Quantification of Cyclobutane Pyrimidine Dimers in Human Epidermal Stem Cells

    Quantification of Cyclobutane Pyrimidine Dimers in Human Epidermal Stem Cells

    The common procedures that are used to quantify cyclobutane pyrimidine dimers (C

    2025-02-20 33
  • HPLC/MS/MS-Based Approaches for Detection and Quantification of Eicosanoids

    HPLC/MS/MS-Based Approaches for Detection and Quantification of Eicosanoids

    Eicosanoids are oxygenated, endogenous, unsaturated fatty acids derived from ara

    2025-02-20 38
  • 初级溶酶体(primary lysosome)

    初级溶酶体(primary lysosome)

    此类溶酶体是刚刚从反面高尔基体形成的小囊泡, 仅含有水解酶类,但无作用底物,外面只有一层单位膜,其中的酶处于非活性状态。如果从细胞的分泌活动考虑,初级溶酶体是一

    2025-02-19 33
  • Assays of Adrenergic Receptors: Including Lipolysis and Binding Measurements

    Assays of Adrenergic Receptors: Including Lipolysis and Binding Measurements

    Adipocytes express the three major types of adrenoceptors (ARs), α1 -, α2 -, and

    2025-02-19 40
  • Role of Endothelial CellCell Junctions in Endothelial Permeability

    Role of Endothelial CellCell Junctions in Endothelial Permeability

    The endothelial barrier separates the inner blood compartment from the surroundi

    2025-02-19 49
  • Purification of Plant Ribosomal DNA: Use of Buoyant Density Centrifugation in Cesium Chloride-Actino

    Purification of Plant Ribosomal DNA: Use of Buoyant Density Centrifugation in Cesium Chloride-Actino

    The general features of isopycnic centrifugation of DNA and the separation of ma

    2025-02-19 36
  • Entrapment of Enzymes and Cells in Poly (2-Hydroxyethyl Methacrylate) Supports

    Entrapment of Enzymes and Cells in Poly (2-Hydroxyethyl Methacrylate) Supports

    Immobilization of enzymes and cells has been widely investigated for many years,

    2025-02-19 43
  • Visualization and Interpretation of Eukaryotic DNA Replication Intermediates In Vivo by Electron Mic

    Visualization and Interpretation of Eukaryotic DNA Replication Intermediates In Vivo by Electron Mic

    The detailed understanding of the DNA replication process requires structural in

    2025-02-19 56
  • Rat Brain Neuropeptidomics: Tissue Collection, Protease Inhibition, Neuropeptide Extraction, and Mas

    Rat Brain Neuropeptidomics: Tissue Collection, Protease Inhibition, Neuropeptide Extraction, and Mas

    Due to the complexity of the mammalian central nervous system, neuropeptidomic s

    2025-02-19 54
  • Generation of Tissue Organoids by Compaction Reaggregation

    Generation of Tissue Organoids by Compaction Reaggregation

    Cellular reaggregation methods are commonly used to generate tissue organoids fo

    2025-02-19 42
  • Amylose Affinity Chromatography of Maltose-Binding Protein: Purification by both Native and Novel Ma

    Amylose Affinity Chromatography of Maltose-Binding Protein: Purification by both Native and Novel Ma

    Maltose-binding protein (MBP) is a carrier protein for high level recombinant pr

    2025-02-19 37
  • Isolation, Propagation, and Characterization of Human Umbilical Cord Perivascular Cells (HUCPVCs)

    Isolation, Propagation, and Characterization of Human Umbilical Cord Perivascular Cells (HUCPVCs)

    Current sources of mesenchymal cells, including bone marrow, fat and muscle, all

    2025-02-19 28
  • Murine Bone Marrow-Derived Macrophages

    Murine Bone Marrow-Derived Macrophages

    The molecular phagocytic lineage comprises, in order of increasing maturity, the

    2025-02-19 24