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游离核糖体(free ribosomes)
在蛋白质合成的全过程中, 结合有mRNA的核糖体都是游离存在的（实际上是与细胞骨架结合在一起的）,不与内质网结合。这种核糖体之所以不与内质网结合, 是因为被合成
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关于LIPO2000转染HepG2细胞的问题
相关专题 总有一种转染方法适合你乡乡问：最近本人在做HepG2细胞的转染，转染的是GFP质粒 ，按照LIPO2000的说明书使用后，发现细胞的死亡很严重，24h
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Using cDNA-Representational Difference Analysis (cDNA-RDA) in Combination with Microarrays to Identi
Small GTPases of the Ras superfamily are molecular switches which cycle between
2025-02-28 58 -
Adult Rodent Spinal Cord-Derived Neural Stem Cells: Isolation and Characterization
Self-renewing multipotent stem/progenitor cells have been isolated from various
2025-02-28 65 -
人脐静脉内皮细胞的体外培养、鉴定与形态观察
相关专题 1. 标本采集 在无菌条件下于健康产妇分娩后立即取新生儿脐带， 挤出脐带血管中的血液，放入装有含青链霉素的PBS液瓶中，2h之内行脐静脉内皮细胞培
2025-02-28 59 -
Viral Transduction of Adipose-Derived Stem Cells
Increasing numbers of regenerative approaches now involve use of adult stem cell
2025-02-28 49 -
Isolation of Microtubules (Bovine Brain)
Isolation of Microtubules (Bovine Brain)LEVEL IIMaterials Freshly removed bovine
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Feeder-Free Culture of hESCs
MEF Conditioned mediumPlate MEFs (p4 or p5) onto gelatin-coated plates at a dens
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Immunofluorescent Localization of Tubulin
Immunofluorescent Localization of TubulinLEVEL IIMaterials Coverslip cultures of
2025-02-28 57 -
Ultrarapid Vitrification of Mouse Oocytes and Embryos
Cryopreservation facilitates long-term storage of gametes and embryos for numero
2025-02-28 50 -
Using the Fluorescent Styryl Dye FM1-43 to Visualize Synaptic Vesicles Exocytosis and Endocytosis in
The styryl dye FM1-43 is a powerful tool to track exocytosis, endocytosis and re
2025-02-28 44 -
Liposome Formulations of Hydrophobic Drugs
Here, we report methods of preparation for liposome formulations containing lipo
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Isolation of DNA Structure-Dependent Checkpoint Mutants in S. pombe
Eukaryotic cells have the ability to influence progression through the cell cycl
2025-02-28 58 -
In Vitro and In Vivo Assays for Studying Histone Ubiquitination and Deubiquitination
Posttranslational histone modifications play important roles in regulating chrom
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小鼠胚胎干细胞培养实验步骤
相关专题以下是根据NIH老年病研究所提供的英文的R1胚胎干细胞培养 protocol整理而成。根据经验作部分修改。1、一般培养-保持胚胎干细胞 处于未分化状态培
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