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红细胞裂解液的配制
红细胞裂解液(Tris-NH4Cl):称取3.735g氯化铵、三羟甲基氨基甲烷(Tris)1.3g加水溶解并稀释至500ml。0.22μm滤膜过滤除菌,4℃保存
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Adipogenic Differentiation of Adipose-Derived Stem Cells
The primary physiological function of adipose-derived stem cells (ASCs) is to di
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Detection of RTK Pathway Activation in Drosophila Using Anti-dpERK Immunofluorescence Staining
In Drosophila , like in other metazoans, receptor tyrosine kinase (RTK) signalin
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非循环式光合磷酸化(non-cyclic photophosphorylation)
在线性电子传递中,光驱动的电子经两个光系统最后传递给NADP ,并在电子传递过程中建立H 质子梯度,驱使ADP磷酸化产生ATP。非循环式电子传递和光合磷酸化的最
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成核反应(nucleation)
在细胞骨架纤维的组装过程中, 构成骨架的基本构件(如微管蛋白、肌动蛋白)在一定的调节下形成一个核心, 这一核心具有指导进一步装配的作用。
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前期(prophase)
前期是有丝分裂的第一期, 该期的主要特征是染色质凝缩成完全相同的两条染色单体连接而成的具有明显特征的染色体。前期发生的主要事件有4种:染色体的凝集、分裂极的确定
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核糖核酸酶P (ribonuclease P)
核糖核酸酶 P 是一种核糖核蛋白, 含有一个单链RNA分子, 长度为375个碱基, 结合一个相对分子质量为20kDa的多肽(119个氨基酸残基)。RNA具有催化
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小鼠白细胞介素8(IL-8)酶联免疫分析(ELISA)
小鼠白细胞介素8 (IL-8) 酶联免疫 分析( ELISA )试剂 盒使用说明书本试剂仅供研究使用 目的:本试剂盒用于测定小鼠血清,血浆及相
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Protein Overexpression in Skeletal Muscle Using Plasmid-Based Gene Transfer to Elucidate Mechanisms
Plasmid DNA electrotransfer is a direct method of gene delivery to skeletal musc
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Functional Identification of Neural Stem Cell-Derived Oligodendrocytes
Directing neural stem cells (NSCs) differentiation towards oligodendroglial cell
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Convenient and Efficient Enrichment of the CD133+ Liver Cells from Rat Fetal Liver as a Source of Li
Although stem cells are commonly isolated by fluorescence-activated cell sorting
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核定位信号(nuclear localization signal, NLS)
核定位信号是另一种形式的信号肽, 可位于多肽序列的任何部分。一般含有 4~8个氨基酸, 且没有专一性, 作用是帮助亲核蛋白进入细胞核。入核信号与导肽的区别在于:
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Platelet Preparation
OUTLINE In order to avoid platelet activation all manipulations must be performe
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FIXATION and DNA Staining for Cell Cycle Analysis
FIXATION and DNA Staining for Cell Cycle AnalysisBackgroundThis method of DNA st
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Neural Differentiation of Human Embryonic Stem Cells
Embryonic stem cells (ESCs) are pluripotent and capable of indefinite self-renew
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