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  • Capillary Electrophoresis of Neutral Carbohydrates: Mono-, Oligosaccharides, Glycosides

    Capillary Electrophoresis of Neutral Carbohydrates: Mono-, Oligosaccharides, Glycosides

    This chapter reports an overview of the recent advances in the analysis of neutr

    2025-02-27 47
  • 反义RNA(antisense RNA)

    反义RNA(antisense RNA)

    反义RNA是指与mRNA互补的RNA分子, 也包括与其它RNA互补的RNA分子。由于核糖体不能翻译双链的RNA,所以反义RNA与mRNA特异性的互补结合, 即抑

    2025-02-27 37
  • Analyzing Mammalian Female Meiosis

    Analyzing Mammalian Female Meiosis

    The goal of this chapter is twofold: First, to acquaint the reader with the prob

    2025-02-27 52
  • 细胞计数和存活测试

    细胞计数和存活测试

    相关专题 1. 原理: 1.1. 计算细胞数目可用血球计数盘或是Coulter counter 粒子计数器自动计数。 1.2. 血球计数盘一般有二个cham

    2025-02-27 46
  • Human Alkaline Phosphatase staining of transgenic embryo/tissue

    Human Alkaline Phosphatase staining of transgenic embryo/tissue

    AP Buffer:100 mM Tris-HCl, pH 9.5100 mM NaCl10 mM MgCl2PTM:PBS0.1% Tween-202 mM

    2025-02-27 49
  • Coimmunoprecipitation on Drosophila Cells in Culture

    Coimmunoprecipitation on Drosophila Cells in Culture

    Coimmunoprecipitation (coIP) provides evidence that two or more proteins can be

    2025-02-27 49
  • 无菌操作注意事项

    无菌操作注意事项

    相关专题 体外培养细胞缺乏抗感染能力,所以防止污染是决定培养成功或失败的首要条件。即便使用设备完善的实验室。若实验者粗心大意,技术操作不规范,也会导致污染。

    2025-02-27 51
  • Cell Proliferation Assay

    Cell Proliferation Assay

    IntroductionLiving (metabolically active) cells reduce tetrazolium salts to colo

    2025-02-27 66
  • Cdc2蛋白(Cdc 2 protein)

    Cdc2蛋白(Cdc 2 protein)

    Cdc2蛋白是裂殖酵母cdc2基因编码的蛋白,分子两34kDa,所以有称为p34cdc2 蛋白。通过对分离的裂殖酵母突变体的研究发现, 没有Cdc2的活性,细胞

    2025-02-27 49
  • 糖原的显示—PAS反应(periodic acid schiff’s reaction)

    糖原的显示—PAS反应(periodic acid schiff’s reaction)

    相关专题 目的要求 通过PAS反应,了解糖原显示的基本原理、方法以及糖原在细胞中的分布。 基本原理 糖原由D-葡萄糖的分支或直链组成,在肝和肌肉最丰富。过碘酸

    2025-02-27 56
  • Evaluation of mTOR-Regulated mRNA Translation

    Evaluation of mTOR-Regulated mRNA Translation

    mTOR, the mammalian target of rapamycin, regulates protein synthesis (mRNA trans

    2025-02-27 43
  • Culture of Endometrial Explants and Peri-implantation Conceptuses to Monitor Synthesis and Secretion

    Culture of Endometrial Explants and Peri-implantation Conceptuses to Monitor Synthesis and Secretion

    Culture of Endometrial Explants and Peri-implantation Conceptuses to Monitor Syn

    2025-02-27 39
  • XTT

    XTT

    相关专题   材料与方法  1. 主要试剂:MTT、XTT、PMS、DMSO (Sigma公司产品),RPMI  1640(Gibco公司),氟尿嘧啶(上海旭

    2025-02-27 46
  • 猴白细胞介素17(IL-17)酶联免疫分析(ELISA)

    猴白细胞介素17(IL-17)酶联免疫分析(ELISA)

    猴 白细胞介素17(IL-17) 酶联免疫 分析( ELISA )试剂 盒使用说明书本试剂仅供研究使用 目的:本试剂盒用于测定猴血清,血浆及相

    2025-02-27 59
  • Chemotaxis Assay

    Chemotaxis Assay

    Chemotaxis Assayby Melissa Swope, 3/12/2000PurposeThe purpose of a chemotaxis as

    2025-02-27 58