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Large-scale Immunocytology
This protocol describes our method for preparing cells for immunofluorescence, i
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Large-Scale, Nonchromatographic Purification of Plasmid DNA
A large-scale approach to the purification of plasmid DNA has been developed tha
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Lentiviral Gene Transduction in Human and Mouse NK Cell Lines
Natural killer (NK) cells play a vital role in the control of cancer and microbi
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Lentiviral Transduction of Immune Cells
Gene transfer into mammalian cells has been of crucial importance for studies de
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Lentivirus-Mediated Antagomir Expression
MicroRNAs (miRNAs) are small non-coding RNAs involved in post-transcriptional ge
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Leucocyte Adhesion Under Haemodynamic Flow Conditions
Vascular endothelial cells (EC) line the luminal side of all blood vessels and a
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Ligands, Cell-Based Models, and Readouts Required for Toll-Like Receptor Action
This chapter details the tools that are available to study Toll-like receptor (T
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Limiting Dilution Analysis of Antigen-Specific T Cells
Limiting dilution analysis (LDA) has been extensively employed as a quantitative
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Limiting Dilution Assay
Limiting dilution assays (LDA) are designed to define an unknown frequency of ef
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Limiting Dilutions
NOTE: The sooner after cell fusion you can do a limiting dilution, the better yo
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Liposomal Adjuvants: Preparation and Formulation with Antigens
Many preclinical and clinical results indicate that liposomal systems can serve
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Liposomes As Immunological Adjuvants and Vaccine Carriers
The need for an effective and safe immunological adjuvant or vaccine carrier tha
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Live Cell ELISA Protocol
Reagents1. DMEM:10% FBS2. DMEM:10% FBS:1% HEPES (DFH)3. 10X Substrate Buffer, pH
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Live Imaging of Dendritic CellTreg Cell Interactions
The decision to launch an immune response is made during the interaction of help
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Live Imaging of LeukocyteEndothelium Interactions
Leukocyte extravasation is a highly dynamic, interactive, and coordinated proces
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