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b-Galactosidase Color Assay for Cultured Cells
b-Galactosidase Color Assay for Cultured Cells 1. For transient expression assay
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Isolation of Oligodendroglial Cells from Cultured Neural Stem/Progenitors
The central nervous system (CNS) is composed of multiple cell types formed throu
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Derivation and Maintenance of Human Embryonic Stem Cells
Since their derivation in 1998, human embryonic stem cells (hESCs) have been the
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Derivation of Mouse Embryonic Stem Cell Lines from Blastocysts Produced by Fertilization and Somatic
This chapter describes a detailed protocol for deriving mouse embryonic stem (ES
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Isolation, Culture and Analysis of Mouse Mammary Epithelial Cells
Limited understanding of the cell biology of the breast and breast cancer hamper
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Peptide Structure Determination by NMR
The difference between peptides and proteins (the subject of Chapter 2 ) is that
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A first step in defining the function of a novel gene is to determine its interactions with other ge
The difference between peptides and proteins (the subject of Chapter 2 ) is that
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Detection and Visualization of Protein Interactions with Protein Fragment Complementation Assays
A first step in defining the function of a novel gene is to determine its intera
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Human Embryonic Stem (ES) Cell Protocols――Splitting Human ES cells on MEFs
based on splitting onto one plateWarm collagenase IV split media to 37 C in a wa
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Vascular Progenitor Cell Mobilization
Blood vessel formation plays a key role in both physiologic and pathologic tissu
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Cytochemical Probes of Cycling and Quiescent Cells Applicable to Flow Cytometry
For convenience the term quiescent cells will be used in this chapter to define
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Poly(Ethylene Glycol) Crosslinked to Albumin as a Support for Enzyme Immobilization
Medical applications of enzymes are found in various areas, such as diagnosis, p
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Quantitative Polymerase Chain Reaction
Quantitative PCR (qPCR) has entered widespread use with the increasing availabil
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Chromosome Spreading and Immunofluorescence Methods in Saccharomyes cerevisiae
Visualization of meiotic chromosomes in the model organism S. cerevisiae has bec
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Fluorescence-Activated Cell Sorting Analysis of Mitochondrial Content, Membrane Potential, and Matri
Fluorescence-activated cell sorting (FACS) permits specific biologic parameters
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