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Determination of mRNA and Protein Expression Patterns in Zebrafish
Optically transparent zebrafish embryos provide an excellent vertebrate model sy
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Live-Cell Fluorescence Imaging of Meiotic Chromosome Dynamics in Schizosaccharomyces pombe
The fission yeast Schizosaccharomyces pombe has provided a useful experimental s
2025-02-14 26 -
Ratiometric Ca2+ Measurements Using the FlexStation Scanning Fluorometer
Many commercial organizations currently use the Fluorometric Imaging Plate Reade
2025-02-14 29 -
Methods to Measure Membrane Potential and Permeability Transition in the Mitochondria During Apoptos
Mitochondrial membrane permeabilization (MMP) constitutes an early event of the
2025-02-14 30 -
Experimental Identification of MicroRNA Targets by Immunoprecipitation of Argonaute Protein Complexe
MicroRNAs (miRNAs) represent a class of small noncoding RNAs that negatively reg
2025-02-14 26 -
Microscopic Techniques for Studying Sperm-Oocyte Interaction During Fertilization and Early Embryoni
The union between sperm and oocyte is species-specific, and the courses of event
2025-02-14 48 -
Immobilization of Enzymes and Cells: Some Practical Considerations
The technology for immobilization of cells and enzymes evolved steadily for the
2025-02-14 44 -
Structural Analysis of Protein-RNA Interactions With Mass Spectrometry
We present a high-resolution mass spectrometric footprinting approach enabling t
2025-02-14 38 -
关于动物血清的常见问题及处理方法
最近在培养细胞中使用胎牛血清,由于发现有沉淀,不知是否变质,在查阅相关文献也没有得到确切的答案.因此不敢再用原来的血清,导致浪费.在这提醒大家做细胞培养时,要注
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Chemical Assays for Proteins
The choice of a protein assay for membranes and membrane proteins is dependent o
2025-02-14 26 -
Generation of Human-Induced Pluripotent Stem Cells (hiPSCs) Using Episomal Vectors on Defined Essent
Human-induced pluripotent stem cells (iPSCs) are an important potential source o
2025-02-14 34 -
Isolation and Culture of Human Colon Epithelial Cells Using a Modified Explant Technique Employing a
Colorectal cancer, originating in tissues of epithelial origin, is one of the mo
2025-02-14 28 -
Introduction to Nucleocytoplasmic Transport: Molecules and Mechanisms
Nucleocytoplasmic transport, the exchange of matter between nucleus and cytoplas
2025-02-14 28 -
Analysis of Biomarkers of Caloric Restriction in Aging Cells
Caloric restriction (CR) has been extensively documented for its profound role i
2025-02-14 31 -
常用封闭剂选择的窍门
相关专题 生物实验中的封闭剂杂交膜上有很多非特异性的蛋白质结合位点，为防止这些位点与抗体结合引起非特异的染色和背景，一般用惰性蛋白质或非离子去污剂封闭膜上的未结
2025-02-14 24
