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Methods for the Isolation and Maintenance of Murine Embryonic Stem Cells
Embryonic stem (ES) cells were first isolated in the 1980s by several independen
2025-02-07 6 -
Screening for MAPK Modulators Using an In-Cell Western Assay
Several essential cellular functions are critically influenced by the Ras-Raf-ME
2025-02-07 8 -
Efficient PCR Production of Single-Stranded DNA Sequencing Templates
The acceptance of polymerase chain reaction (PCR) as a routine method in molecul
2025-02-07 10 -
酵母遗传学方法3:RNA的分离
酵母 RNA 的分离 1 )总酵母 RNA 的分离 1. 在每毫升培养物中加入 50 μ g 环乙酰亚胺,在 30 ℃下振荡 15 分钟。此步亦可省略,但是有人
2025-02-07 11 -
Differentiation of Circulating Monocytes into Fibroblast-Like Cells
Monocytes are produced in the bone marrow and enter the blood. They generally le
2025-02-07 9 -
二甲基亚砜的用途是什么
二甲基亚砜(DMSO)是一种多功能的有机溶剂,因其独特的物理化学性质而在多个领域得到了广泛应用。以下是一些主要用途:1. 溶剂DMSO 是一种极好的极性溶剂,能
2025-02-06 844 -
Embryonic Stem Cells as a Model for Studying Osteoclast Lineage Development
Osteoclasts are cells specialized to resorb bone ( 1 , 2 ). They originate from
2025-02-06 14 -
Maintenance of Cell Culture
Maintenance of Cell CultureAuthor: Nanci DonackiSource: Contributed by Nanci Don
2025-02-06 11 -
大鼠氧化低密度脂蛋白(OxLDL)酶联免疫分析(ELISA)
大鼠 氧化低密度脂蛋白(OxLDL) 酶联免疫分析( ELISA )试剂盒使用说明书本试剂仅供研究使用 目的:本试剂盒用于测定大鼠血清,血浆及相
2025-02-06 13 -
Biochemical Characterization of a Testis-Predominant Isoform of N-Alpha Acetyltransferase
N-alpha protein acetylation, catalyzed by N-alpha acetyltransferase complex, is
2025-02-06 9 -
A Stochastic PCR Approach for RNA Quantification in Multiple Samples
When studying the effect of various treatments on gene expression in humans, one
2025-02-06 10 -
Quantitative Analysis of Human DNA Sequences by PCR and Solid-Phase Minisequencing
The PCR technique provides highly specific and sensitive means for analyzing nuc
2025-02-06 14 -
FACS Analysis of the Planarian Stem Cell Compartment as a Tool to Understand Regenerative Mechanisms
Planarians provide a relatively simple model system in which to study stem cell
2025-02-06 11 -
Culture Systems: Single Step
Culture media to support development of zygotes to the blastocyst stage is based
2025-02-06 8 -
Fractionation of the Coxiella burnetii Parasitophorous Vacuole
Coxiella burnetii is a bacterial obligate intracellular pathogen that replicates
2025-02-06 14