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  • Designing, Testing, and Validating a Microarray for Stem Cell Characterization

    Designing, Testing, and Validating a Microarray for Stem Cell Characterization

    Microarray technology is a powerful tool that allows for simultaneous assessment

    2025-02-06 13
  • Spermine Synthase Deficiency Resulting in X-Linked Intellectual Disability (SnyderRobinson Syndrome)

    Spermine Synthase Deficiency Resulting in X-Linked Intellectual Disability (SnyderRobinson Syndrome)

    Polyamines, small positively charged molecules, are vital for cell proliferation

    2025-02-06 11
  • 基因扩增(gene amplification)

    基因扩增(gene amplification)

    细胞内选择性复制DNA, 产生大量的拷贝。如两栖类卵母细胞在发育的早期,rRNA基因的数量扩增到1000多倍。基因扩增是通过形成几千个核进行的,每个核里含有几百

    2025-02-06 10
  • Measurement of Changes in Apoptosis and Cell Cycle Regulatory Kinase Cdk2

    Measurement of Changes in Apoptosis and Cell Cycle Regulatory Kinase Cdk2

    Many cell cycle regulatory proteins have been shown to be able to regulate cell

    2025-02-06 12
  • Quantitative Analysis of JAK Binding Using Isothermal Titration Calorimetry and Surface Plasmon Reso

    Quantitative Analysis of JAK Binding Using Isothermal Titration Calorimetry and Surface Plasmon Reso

    Janus Kinases (JAKs) are the key effector kinases that initiate intracellular si

    2025-02-06 11
  • Isolation, Expansion, and Characterization of Human Islet-Derived Progenitor Cells

    Isolation, Expansion, and Characterization of Human Islet-Derived Progenitor Cells

    Islet transplantation is a widely accepted and practiced cell replacement therap

    2025-02-06 10
  • Rubidium Efflux as a Tool for the Pharmacological Characterisation of Compounds with BK Channel Open

    Rubidium Efflux as a Tool for the Pharmacological Characterisation of Compounds with BK Channel Open

    This chapter describes a method of assaying rubidium (Rb+ ) efflux as a measure

    2025-02-06 9
  • ECM Degradation Assays for Analyzing Local Cell Invasion

    ECM Degradation Assays for Analyzing Local Cell Invasion

    Proteolytic degradation of extracellular matrix (ECM) is a critical step during

    2025-02-06 14
  • Analyzing Import Intermediates of Mitochondrial Proteins by Blue Native Gel Electrophoresis

    Analyzing Import Intermediates of Mitochondrial Proteins by Blue Native Gel Electrophoresis

    Blue native gel electrophoresis (BNGE) is a powerful tool for analyzing native p

    2025-02-06 19
  • Managing Apheresis Complications During the Hematopoietic Stem Cell Collection

    Managing Apheresis Complications During the Hematopoietic Stem Cell Collection

    Adverse reactions may occur during the Hematopoietic Progenitor Cells via Aphere

    2025-02-06 11
  • Isolation of Adult Mouse Stem Keratinocytes Using Magnetic Cell Sorting (MACS)

    Isolation of Adult Mouse Stem Keratinocytes Using Magnetic Cell Sorting (MACS)

    The functional and molecular characterization of mouse epidermal stem cells (SC)

    2025-02-06 9
  • A Historical Perspective of the EGF Receptor and Related Systems

    A Historical Perspective of the EGF Receptor and Related Systems

    Since the isolation of epidermal growth factor (EGF) from mouse submaxillary gla

    2025-02-06 11
  • Cysteinyl-Tagging of Integral Membrane Proteins for Proteomic Analysis Using Liquid Chromatography-T

    Cysteinyl-Tagging of Integral Membrane Proteins for Proteomic Analysis Using Liquid Chromatography-T

    Membrane proteomic analysis is of considerable interest due to the role of recep

    2025-02-06 8
  • 核纤层(lamina)

    核纤层(lamina)

    在与核质相邻的核膜内表面有一层厚30~160nm 网络状蛋白质, 叫核纤层, 对核被膜起支撑作用。核纤层由3种分子量为6~7万道尔顿的多肽亚单位α、β、γ所组成

    2025-02-06 10
  • 磷脂交换蛋白(phospholipid exchang proteins, PEP)

    磷脂交换蛋白(phospholipid exchang proteins, PEP)

    PEP是一种水溶性的载体蛋白,可以在不同的膜结合细胞器之间转移磷脂。转移的过程是: PEP首先与磷脂分子结合, 形成水溶性的复合物进入细胞质基质中, 通过自由扩

    2025-02-06 12