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Studying Fertilization in Cell-Free Extracts: Focusing on Membrane/Lipid Raft Functions and Proteomi
Xenopus oocytes, eggs, and embryos serve as an ideal model system to study sever
2025-02-12 25 -
Cryopreservation of Mammalian Embryos: Slow Cooling
The benefits of banking mammalian embryos for long-term storage have long been r
2025-02-12 25 -
Derivation, Maintenance, and Induction of the Differentiation In Vitro of Equine Embryonic Stem Cell
We describe here the isolation and maintenance of pluripotent embryonic stem (ES
2025-02-12 19 -
Localization of Zona Pellucida Receptors on Live Sperm by Fluorophore-Conjugated Solubilized Zona Pe
The zona pellucida is an extracellular glycoprotein coat that surrounds the oocy
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Minimization of Proteins by Random Fragmentation and Selection
Protein-protein interactions are involved in most biological processes and are a
2025-02-12 20 -
Cytological Analysis of Meiosis in Fixed Drosophila Ovaries
Methods are described to analyze two different parts of the Drosophila ovary, wh
2025-02-12 20 -
Isolation and Purification of Plant Nucleic Acids: Genomic and Chloroplast DNA
The use of molecular protocols has expanded into virtually all branches of plant
2025-02-12 19 -
Hoechst 33342/PI双染色法
1．悬浮生长的细胞在培养状态下加入Heochst 33342，终浓度为1μg/ml；帖壁生长的细胞用含有0.02％EDTA的0.25％胰蛋白酶消化成单细胞悬液，
2025-02-12 23 -
体内肝损伤模型（酒精）
一、材料 纯系SD雄性大鼠，体重180g～200 g，由浙江大学医学院实验动物中心提供。食用酒精选用北京酿酒总厂出品的56度红星二锅头。 二、方法 将大鼠随机分
2025-02-12 23 -
293T细胞的一点培养心得
相关专题 293细胞293T细胞差点被我养死，幸好我又将它起死回生，一点心得。细胞传代：当细胞在细胞培养 瓶内长满达到80%时就要传代，一传二，24小时后再传代
2025-02-12 20 -
Adrenal chromaffin granule (chromaffin vesicle) preparation
Adrenal chromaffin granule (chromaffin vesicle) preparationIntroduction. This pr
2025-02-12 17 -
人(ASD)酶联免疫分析
人 (ASD) 酶联免疫分析试剂盒使用说明书本试剂仅供研究使用 目的：本试剂盒用于测定人血清，血浆及相关液体样本中雄烯二酮(ASD) 的含量。实
2025-02-12 21 -
Lacrimal Experimental Protocol (4/4/94)
Lacrimal Experimental Protocol (4/4/94)Day Before:1. Prepare (6) 50 ml conicals
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PCR of the Ribosomal DNA Intergenic Spacer Regions as a Method for Identifying Mosquitoes in the Ano
Six morphologically indistinguishable but genetically and behaviorally distinct
2025-02-12 22 -
Enrichment of PBMCs with monocytes (The Science Advisory Board)
Description This protocol is used in our lab to reduce the costs of the cell sor
2025-02-12 25
