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Water-Soluble Cationic Methacrylate Polymers for Nonviral Gene Delivery
The aim of gene therapy is to treat inherited or acquired genetic deficiencies (
2025-02-11 18 -
In Situ Hybridization to Polytene Chromosomes of Drosophila melanogasterand Other Dipteran Species
In situ hybridization of nucleotide sequence to Drosophila melanogaster interpha
2025-02-11 29 -
Analysis of Hyaluronan Using Biotinylated Hyaluronan-Binding Proteins
In this chapter, we describe the preparation and use of b-PG, a biotinylated com
2025-02-11 19 -
Assessing In Vivo MicroRNA Function in the Germline Stem Cells of the Drosophila Ovary
A more complete understanding of the biology of adult stem cells could yield imp
2025-02-11 20 -
Analysis of Gene Expression in Skin Using Laser Capture Microdissection
Gene expression analysis is a useful tool to study the molecular mechanisms unde
2025-02-11 29 -
Fluorescence In Situ Hybridization for Molecular Cytogenetic Analysis in Filamentous Fungi
Fluorescence in situ hybridization (FISH) is a powerful technology for studying
2025-02-11 25 -
Immunofluorescence Staining of Spindles, Chromosomes, and Kinetochores in Human Oocytes
Understanding how human oocytes execute chromosome segregation is of paramount i
2025-02-11 22 -
Free-Hand Bisection of Mouse Oocytes and Embryos
Mouse oocytes and zygotes are semitransparent and large cells approximately 80 μ
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SOLID TUMOR CULTURES FOR CHROMOSOME ANALYSIS
I. Purpose:A. Samples of solid tumors or lymph nodes may be sent from patients w
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Measurement of Apoptotic Cell Clearance In Vitro
The phagocytic clearance of apoptotic cells is a highly efficient and non-phlogi
2025-02-11 26 -
Adenoviral Transduction of Mesenchymal Stem Cells
Intact or genetically manipulated mesnechymal stem cells (MSCs) are being consid
2025-02-11 27 -
Generation of Amplified RNAs and cDNA Libraries from Single Mammalian Cells
With the near completion of the human genome sequencing effort, it is now possib
2025-02-11 23 -
Linear-After-The-Exponential Polymerase Chain Reaction and Allied Technologies: Real-Time Detection
Accurate detection of gene sequences in single cells is the ultimate challenge t
2025-02-11 29 -
Essentials of Recombinase-Based Genetic Fate Mapping in Mice
Fate maps, by defining the relationship between embryonic tissue organization an
2025-02-11 25 -
Assessing Chronological Aging in Bacteria
Bacteria, which are often considered as avid reproductive organisms under consta
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