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EMSA凝胶迁移实验常见问题与注意事项
1.什么是凝胶迁移或电泳迁移率实验?凝胶迁移或电泳迁移率实验(EMSA)是一种研究DNA结合蛋白和其相关的DNA结合序列相互作用的技术,可用于定性和定量分析。这
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石蜡切片实验介绍
石蜡切片(paraffin section)是组织学常规制片技术中最为广泛应用的方法。石蜡切片不仅用于观察正常细胞组织的形态结构,也是病理学和法医学等学科用以研
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石蜡切片制作步骤
石蜡切片制作第一步:取材应根据要求选取材料来源及部位。材料必须新鲜,搁置时间过久则产生蛋白质分解变性,导致细胞自溶及细菌的滋生,而不能反映组织活体时的形态结构。
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石蜡切片制作过程中的注意事项
石蜡切片取材时要注意:(1)取材动作要迅速,不宜拖太久,以免组织细胞的成分、结构等发生变化。(2)切片材料应根据需要观察的部位进行选择,尽可能不要损伤所需要的部
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石蜡切片制作常见问题与解决方法【上】
问题一、切片上卷且不能形成连续蜡带原因:1、切片刀不够锋利。2、切片刀与蜡块的角度不当。3、’蜡块四周留蜡边太少。4、石蜡硬度过大,黏度不够。解决方法:1、更换
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石蜡切片制作常见问题与解决方法【下】
问题一、切片皱褶太多且不能展开原因:1、蜡块太软或室温过高。2、切片刀上粘有残余的石蜡或刀口不干净。3、组织浸蜡时间不够或脱水、透明不够。4、切片刀不锋利。解决
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Microarray Data Analysis in Studies of Membrane Transporters
The newly developed microarray technology has made it possible to simultaneously
2025-02-11 28 -
Gene Expression Profiling of Mouse Epidermal Keratinocytes
DNA microarray technology is a powerful tool for genome-wide gene expression ana
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Stable Integration of Vectors at High Copy Number for High-Level Expression in Animal Cells
The efficient production of proteins via expression of recombinant genes in cell
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Solid Phase Assays for Studying ECM Protein-Protein Interactions
Solid-phase assays provide a simple, rapid and robust method for the analysis of
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Quantification of Transendothelial Migration Using Three-Dimensional Confocal Microscopy
Migration of cells across endothelial barriers, termed transendothelial migratio
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Cryoelectron Microscopy of Vitreous Sections: A Step Further Towards the Native State
Nuclear architecture has been investigated intensively by various electron micro
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Exploiting the Liberation of Zn2+ to Measure Cell Viability
Zn2+ ions are a critical component of cellular machinery. The ion is required fo
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将构建的真核表达载体转染进HEK293细胞的方法
相关专题 293细胞 总有一种转染方法适合你问:我是新手,我想将一个构建的真核表达载体转染进HEK293细胞 中,有人说要先将质粒线性化,也有人说不用,不知道到
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Molecular Cytogenetic Applications in Diagnostics and Research: An Overview
Molecular cytogenetics is a new branch of chromosome research that is just is ju
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