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Analysis of DNA Methylation Profiles in Preimplantation Embryos Using Bisulfite Mutagenesis
For developmental competence of mammalian embryos, dynamic epigenetic changes to
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Electroporation of ES cells
Cells are routinely passaged two days prior to electroporating. Usually one 10 c
2025-01-22 15 -
Refolding of TIMP-2 from Escherichia coli Inclusion Bodies
E. coli is a convenient host in which to express recombinant proteins. The techn
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Human Embryo Culture Media Comparisons
Every program of assisted reproduction strives to maximize pregnancy outcomes fr
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Preparation of Intact, Highly Purified Phagosomes from Dictyostelium
Phagocytosis plays a fundamental role in the immune system for the defense again
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Solid Tissue Dispersal for Cytokinetic Analyses
The dispersal of solid tissues into a single-cell suspension has become an integ
2025-01-22 14 -
RNA Extraction from Formalin-Fixed and Paraffin-Embedded Tissues
Fixed and paraffin-embedded tissues from pathology department archives can be av
2025-01-22 12 -
Transient Expression of Epitope-Tagged Proteins in Mammalian Cells
Before the advent of molecular methods to tag proteins, the visualization of pro
2025-01-22 12 -
Automated Adherent Human Cell Culture (Mesenchymal Stem Cells)
Human cell culture processes developed at research laboratory scale need to be t
2025-01-22 11 -
染色体CBG标本的制备
相关专题 一、原理 异染色质在整个分裂间期及分裂期都是浓缩的，因而其大小较为恒定。它们通常位于着丝粒 附近，或在染色体臂上呈现“团块”，这些染色质主要由C显
2025-01-22 11 -
Urothelial Cell Culture: Stratified Urothelial Sheet and Three-Dimensional Growth of Urothelial Stru
Urothelial cells line the urinary tract, including the renal pelvis, ureters, bl
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In Situ Biosynthesis of Peptide Arrays
Polypeptide and protein arrays enable high-throughput screening capabilities for
2025-01-22 12 -
细胞培养染色体显示
1 ） 传代培养细胞染色体显示法1 ．培养细胞：取处于指数生长期、用较大瓶皿培养的、 80 ％～ 90 ％汇合单层培养细胞。2 ．加秋水仙素：使用最终浓度为 0
2025-01-22 11 -
Physical Properties of the Nucleus Studied by Micropipette Aspiration
Understanding the physical properties of the cell nucleus is critical for develo
2025-01-22 9 -
Microscopic Analysis of the Adult Drosophila Retina Using Semithin Plastic Sections
The regular appearance and the repetitive nature of the Drosophila eye, consisti
2025-01-22 10
