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New Protocol for Lentiviral Vector Mass Production
Multiplasmid transient transfection is the most widely used technique for the ge
2025-02-10 24 -
Analyzing mRNA Localization to the Endoplasmic Reticulum via Cell Fractionation
The partitioning of secretory and membrane protein-encoding mRNAs to the endopla
2025-02-10 30 -
The Genome of Dictyostelium discoideum
The Dictyostelium discoideum genome has been sequenced, assembled and annotated
2025-02-10 23 -
Methods for Assessing Effects of Wnt/-Catenin Signaling in Senescence of Mesenchymal Stem Cells
Mesenchymal stem cells (MSCs) represent a main population of stem cells and can
2025-02-10 23 -
Imaging Cells Within 3D Cell-Derived Matrix
Much of what is known about the mechanisms regulating cell adhesion and migratio
2025-02-10 23 -
Purification of Proteins Using Displacement Chromatography
Displacement chromatography has several advantages over the nonlinear elution te
2025-02-10 20 -
Isolation and Generation of Neurosphere Cultures from Embryonic and Adult Mouse Brain
Neural stem cells are defined as cells that either gives rise to or derives from
2025-02-10 19 -
Site-Directed Mutagenesis to Study the StructureFunction Relationships of Ion Channels
Ion channels mediate a wide variety of physiological processes by forming small
2025-02-10 22 -
Preparation of Immobilized Subunits of a Multisubunit Enzyme
It is well established that many intracellular enzymes are oligomeric with dimer
2025-02-10 22 -
MTT Cell Proliferation Assay
MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay, first
2025-02-10 28 -
Gene Expression Analyzed by Ribonuclease Protection Assay
The ribonuclease (RNase) protection assay provides a highly sensitive method for
2025-02-10 28 -
Methods for Gene Expression Profiling in Dermatology Research Using DermArray Nylon Filter DNA Micro
Here we present methods of gene expression profiling using nylon filter deoxyrib
2025-02-10 34 -
Serial Section Immunoelectron Microscopy of Algal Cells
Electron microscopy when combined with immunogold labeling provides a 2D image o
2025-02-10 29 -
Assessment of Development and Chemotaxis in Dictyostelium discoideum Mutants
Studies using the social amoeba Dictyostelium discoideum have greatly contribute
2025-02-10 28 -
中心粒(centrioles)
中心体的主要结构, 成对存在, 即一个中心体含有一对中心粒，且互相垂直形成"L"形排列。中心粒直径为0.2μm. 长为0.4μm，是中空的短
2025-02-10 29
