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Analyses of ProteinProtein Interactions by In Vivo Photocrosslinking in Budding Yeast
Recent development of methods for genetic incorporation of unnatural amino acids
2025-01-18 7 -
Single Cell Enzymatic Dissociation of Human Embryonic Stem Cells: A Straightforward, Robust, and Sta
The routine culture and expansion of human embryonic stem (hES) cells has been a
2025-01-18 11 -
Cloning by Limiting Dilution of Hybridoma
Cloning by Limiting Dilution of HybridomaAuthor: Nanci DonackiSource: Contribute
2025-01-18 9 -
Study of Apoptosis In Vitro Using the Xenopus Egg Extract Reconstitution System
It was first shown by Newmeyer and colleagues in the 1990s that the molecular ev
2025-01-18 13 -
Derivation and Characterization of Neuronal Precursors and Dopaminergic Neurons From Human Embryonic
Dopaminergic neurons of human origin have many potential research applications s
2025-01-18 13 -
Procedures for Derivation and Characterisation of Human Embryonic Stem Cells from Odense, Denmark
In 1998, a development occurred in stem cell biology with the first report of th
2025-01-18 8 -
Multicolor Whole-Mount In Situ Hybridization
In situ hybridization is a procedure that allows the detection of the site(s) of
2025-01-18 9 -
Measurement of Cholesterol in Membranes
Cholesterol is an amphipathic molecule of great importance in biology. It can be
2025-01-18 13 -
Grafting Hensens Node
Soon after Spemann and Mangold’s (1 ) famous demonstration in 1924 that the dors
2025-01-18 12 -
Culture of Human Normal Brain and Malignant Brain Tumors for Cellular, Molecular, and Pharmacologica
Human brain neoplasms comprise a highly heterogeneous and biologically diverse g
2025-01-18 7 -
Refining Efficacy: Allosterism and Bias in G Protein-Coupled Receptor Signaling
Receptors on the surface of cells function as conduits for information flowing b
2025-01-18 10 -
Photoactivatable (Caged) Fluorescein as a Cell Tracer for Fate Mapping in the Zebrafish Embryo
Classical fate mapping approaches in any embryo typically involve labeling of si
2025-01-18 8 -
F2-Isoprostanes: Sensitive Biomarkers of Oxidative Stress In Vitro and In Vivo: A Gas Chromatography
A gas chromatography-mass spectrometric method was developed that allowed the ac
2025-01-18 8 -
Recombinases and Their Use in Gene Activation, Gene Inactivation, and Transgenesis
The site-specific recombinase FLP is used in Drosophila to precisely manipulate
2025-01-18 9 -
Poly(ADP-ribosyl)ation Pathways in Mammals: The Advantage of Murine PARG Null Mutation
Studies suggest that inhibiting the hydrolysis of poly(ADP-ribose) (PAR) by targ
2025-01-18 13
