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Posttranscriptional Regulation of Gene Expression in Epithelial Cells by Polyamines
In addition to regulating gene transcription, polyamines also potently modulate
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The Calvarial Injection Assay
This chapter describes the calvarial injection method, whereby the effect of a s
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Epidermis-Targeted Gene Transfer Using In Vivo Electroporation
The skin is an important target for gene transfer because of its easy accessibil
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Skeletal Muscle Satellite Cells: Background and Methods for Isolation and Analysis in a Primary Cult
Repair of adult skeletal muscle depends on satellite cells, myogenic stem cells
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愈伤组织(callus， culli)
植物受创伤后，在伤面新生的组织称为愈伤组织。其原因是由于受创伤的刺激后，伤面附近的生活组织恢复了分裂机能，加速增生而将伤面愈合。在植物组织培养中的愈伤组织是指植
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Monitoring Circadian Rhythms in Arabidopsis thaliana Using Luciferase Reporter Genes
Arabidopsis thaliana is internationally adopted as the model plant species for m
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Generation of Human Melanocytes from Induced Pluripotent Stem Cells
The discovery of human induced pluripotent stem cells (iPSCs) has provided a mod
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Isolation and Culture of Porcine Neural Progenitor Cells from Embryos and Pluripotent Stem Cells
The isolation and culture of neural progenitor cells (NPCs) from pluripotent ste
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Feeder-Layer Free Culture System for Human Embryonic Stem Cells
Human embryonic stem cells (hESCs) are pluripotent stem cells derived from the i
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A Simple Method for Site-Specific Mutagenesis that Leaves the Rest of the Template Unaltered
Site-directed mutagenesis is an important component of modern molecular biology.
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Media Composition: Salts and Osmolality
The main components of embryo culture media are salts, which dissociate into the
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Bioreactor Cultivation of Functional Bone Grafts
The clinical demand for functional tissue-engineered bone grafts to regenerate b
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Genotyping of Mycobacterium tuberculosis Clinical Isolates Using IS6110-Based Restriction Fragment L
A number of phylogenetic studies of Mycobacterium tuberculosis have suggested a
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Trophoblast Differentiation: An In Vitro Model for Trophoblast Giant Cell Development
Trophoblast cells are situated between maternal and embryonic compartments and e
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Direct Sequencing of PCR Products with DNA-Binding Proteins
The polymerase chain reaction (PCR) has become widely established as a powerful
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