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Efficient Generation of Dopamine Neurons from Human Embryonic Stem Cells
In this chapter, we introduce a co-culture protocol for human embryonic stem (hE
2025-02-19 25 -
Directed Differentiation of Neural-stem cells and Subtype-Specific Neurons from hESCs
We describe a chemically defined protocol for efficient differentiation of human
2025-02-19 23 -
Visualization of Sperm Accessory Structures in the Mammalian Spermatids, Spermatozoa, and Zygotes by
Mammalian fertilization is traditionally viewed as a process during which the fe
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Cell Synchronization by Inhibitors of DNA Replication Induces Replication Stress and DNA Damage Resp
Cell synchronization is often achieved by inhibition of DNA replication. The cel
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Sequencing of (dA:dT) Cloned Mixed PCR Products from Microbial Populations
Because only between 1 and 10% of bacteria present in soil and aquatic environme
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A High-Throughput Mammalian Cell-Based Transient Transfection Assay
In eukaryotic organisms gene expression is regulated through a variety of upstre
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锚定蛋白(ankyrin)
又称2.1蛋白。锚定蛋白是一种比较大的细胞内连接蛋白, 每个红细胞约含10万个锚定蛋白，相对分子质量为215,000。锚定蛋白一方面与血影蛋白相连, 另一方面与
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Stepwise Differentiation from Nave State Pluripotent Stem Cells to Functional Primordial Germ Cells
A group of pluripotent cells appearing during mammalian embryogenesis is the sou
2025-02-19 24 -
An In Situ Hybridization Technique to Detect Low-Abundance Slug mRNA in Adherent Cultured Cells
In this chapter, we describe a simple and relatively rapid technique for detecti
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Vertical Arrays: Microarrays of Complex Mixtures of Nucleic Acids
Vertical arrays are microarrays that have complex mixtures of nucleic acids as a
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Fusion Protocol for the Production of Mouse Hybridomas
Hybridoma technology makes it possible to produce almost unlimited amounts of mo
2025-02-19 23 -
Use of RNA Interference to Investigate Cytokine Signal Transduction in Pancreatic Beta Cells
Type 1 diabetes (T1D) is a chronic autoimmune disease characterized by immune in
2025-02-19 23 -
An Assay for Human Chemosignals
Like all mammals, humans use chemosignals. Nevertheless, only few such chemosign
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Pluripotency: Capacity for In Vitro Differentiation of Undifferentiated Embryonic Stem Cells
Embryonic stem (ES) cells, the pluripotent cells of early embryos have been succ
2025-02-19 25 -
Isolation of Protein Complexes Involved in Mitosis and Cytokinesis from Drosophila Cultured Cells
The identification of all the individual components that constitute the plethora
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