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细胞学技术的一般环节
一、纺锤体阻断剂（Spindle inhibitor） 在有丝分裂过程中，随着纺锤丝的形成，染色体被牵引到一起难以观察其形态。纺锤体的形成在于细胞质和纺锤体成分
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DNA Fragmentation Assays for Apoptosis
Protocol I: Triton X-100 Lysis BufferIn 96 flat-wells plate, incubate 4x10 6 tar
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Polyamine Transport Systems in Mammalian Cells and Tissues
Polyamine transport plays an important role in the homeostatic regulation of the
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Detection of c-myc Amplification in Formalin-Fixed Paraffin-Embedded Tumor Tissue by Chromogenic In
In situ hybridization (ISH) allows evaluation of genetic abnormalities, such as
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In Vitro and Ex Vivo Gene Delivery to Cells by Electroporation
Electroporation generally refers to the technique of permeabilizing cell membran
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Introduction: Why Analyze Single Cells
Powerful methods in molecular biology are abundant; however, in many fields incl
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Methods for Homologous Recombination in Drosophila
We present detailed protocols for two methods of gene targeting in Drosophila .
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Serum-Free and Feeder-Free Culture Conditions for Human Embryonic Stem Cells
Human embryonic stem cells (hESCs) are pluripotent cells derived from the embryo
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Subtelomeric and/or Subcentromeric Probe Sets
Aside from microscopically visible chromosomal rearrangements, numerous cryptic
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Sedimentation Velocity Analytical Ultracentrifugation in Hydrogenated and Deuterated Solvents for th
This chapter is a step-by-step protocol for setting up, realizing, and analyzing
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小鼠重组胰岛素抗体酶联免疫分析（ELISA）
小鼠重组胰岛素抗体 酶联免疫 分析（ ELISA ）试剂 盒使用说明书本试剂仅供研究使用 目的：本试剂盒用于测定小鼠血清，血浆及相关液体样本中
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Oocytes as an Expression System for Studying Receptor/Channel Targets of Drugs and Pesticides
The Xenopus laevis oocyte offers one of the most convenient expression systems f
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Recycling Tubulin
Recycling Tubulin We "recycle" tubulin fractions stored at -80¡C after
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Overview: Imaging in the Study of Integrins
Integrins play critical adhesion and signaling roles during development, wound h
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Use of Disulfide Cationic Lipids in Plasmid DNA Delivery
Gene therapy provides a paradigm of the treatment of human diseases. The ultimat
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