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Preparation of Rat Liver Cell Cytosol
These protocols should yield enough cytosol and organelles for 1-200 MT/Organell
2025-02-18 50 -
Mass Measurements of Cyclic AMP Formation by Radioimmunoassay, Enzyme Immunoassay, and Scintillation
Adenosine 3′,5′-cyclic monophosphate (cAMP) is involved in a myriad of normal an
2025-02-18 54 -
Determination of Tetrahydrozoline in Urine and Blood Using Gas Chromatography-Mass Spectrometry (GC-
Tetrahydrozoline, a derivative of imidazoline, is widely used for the symptomati
2025-02-18 66 -
原生质理论(protoplasm theory)
1861年由舒尔策(Max Schultze)提出, 认为有机体的组织单位是一小团原生质,这种物质在一般有机体中是相似的,并把细胞明确地定义为:“细胞是具有细胞
2025-02-18 71 -
Measurement of Phospholipase C by Monitoring Inositol Phosphates Using [3H]Inositol-Labeling Protoco
Hormones, neurotransmitters, chemoattractants, and growth factors all elicit int
2025-02-18 54 -
Spermatogonial Stem Cell Transplantation, Testicular Function, and Restoration of Male Fertility in
Mammalian spermatogonial stem cells, sometimes called male germline stem cells,
2025-02-18 58 -
Production of Human and Murine Eosinophils In Vitro and Assay for Eosinophil Differentiation Factors
Bone marrow cells from a number of animal species have been used extensively in
2025-02-18 60 -
Formation of Embryoid Bodies from Human Pluripotent Stem Cells Using AggreWell Plates
Many human embryonic stem (hES) and induced pluripotent stem (hiPS) cell differe
2025-02-18 63 -
Isolation and Purification of Functionally Intact Mitochondria from Plant Cells
The isolation of mitochondria from plant cells that display similar biochemical
2025-02-18 70 -
S期促进因子(S phase-promoting factor,SPF)
与MPF相似,芽殖酵母的S期促进因子(S phase-promoting factor,SPF),也是异质二聚体, 一个是Cdc28, 另一个是在G1期起作用的
2025-02-18 46 -
PEG介导的动物细胞融合技术
相关专题 细胞融合(cell fusion)或细胞杂交(cell hybridization)是指真核细胞通过介导和培养,两个或多个细胞合并成一个双核或多核细
2025-02-18 91 -
细胞分泌(cell secretion)
动物细胞和植物细胞将在粗面内质网上合成而又非内质网组成部分的蛋白和脂通过小泡运输的方式经过高尔基体的进一步加工和分选运送到细胞内相应结构、细胞质膜以及细胞外的过
2025-02-18 65 -
Examination of the Brain Mitochondrial Lipidome Using Shotgun Lipidomics
Contamination from subcellular organelles and myelin has hindered attempts to ch
2025-02-18 60 -
Dorsal Axis Duplication as a Functional Readout for Wnt Activity
The easy accessibility, distinctive features of early cleavage stage embryos and
2025-02-18 52 -
High-Throughput FACS Method for Directed Evolution of Substrate Specificity
Directed evolution is a powerful technology for the isolation of enzyme variants
2025-02-18 64