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  • Preparation of Rat Liver Cell Cytosol

    Preparation of Rat Liver Cell Cytosol

    These protocols should yield enough cytosol and organelles for 1-200 MT/Organell

    2025-02-18 50
  • Mass Measurements of Cyclic AMP Formation by Radioimmunoassay, Enzyme Immunoassay, and Scintillation

    Mass Measurements of Cyclic AMP Formation by Radioimmunoassay, Enzyme Immunoassay, and Scintillation

    Adenosine 3′,5′-cyclic monophosphate (cAMP) is involved in a myriad of normal an

    2025-02-18 54
  • Determination of Tetrahydrozoline in Urine and Blood Using Gas Chromatography-Mass Spectrometry (GC-

    Determination of Tetrahydrozoline in Urine and Blood Using Gas Chromatography-Mass Spectrometry (GC-

    Tetrahydrozoline, a derivative of imidazoline, is widely used for the symptomati

    2025-02-18 66
  • 原生质理论(protoplasm theory)

    原生质理论(protoplasm theory)

    1861年由舒尔策(Max Schultze)提出, 认为有机体的组织单位是一小团原生质,这种物质在一般有机体中是相似的,并把细胞明确地定义为:“细胞是具有细胞

    2025-02-18 71
  • Measurement of Phospholipase C by Monitoring Inositol Phosphates Using [3H]Inositol-Labeling Protoco

    Measurement of Phospholipase C by Monitoring Inositol Phosphates Using [3H]Inositol-Labeling Protoco

    Hormones, neurotransmitters, chemoattractants, and growth factors all elicit int

    2025-02-18 54
  • Spermatogonial Stem Cell Transplantation, Testicular Function, and Restoration of Male Fertility in

    Spermatogonial Stem Cell Transplantation, Testicular Function, and Restoration of Male Fertility in

    Mammalian spermatogonial stem cells, sometimes called male germline stem cells,

    2025-02-18 58
  • Production of Human and Murine Eosinophils In Vitro and Assay for Eosinophil Differentiation Factors

    Production of Human and Murine Eosinophils In Vitro and Assay for Eosinophil Differentiation Factors

    Bone marrow cells from a number of animal species have been used extensively in

    2025-02-18 60
  • Formation of Embryoid Bodies from Human Pluripotent Stem Cells Using AggreWell Plates

    Formation of Embryoid Bodies from Human Pluripotent Stem Cells Using AggreWell Plates

    Many human embryonic stem (hES) and induced pluripotent stem (hiPS) cell differe

    2025-02-18 63
  • Isolation and Purification of Functionally Intact Mitochondria from Plant Cells

    Isolation and Purification of Functionally Intact Mitochondria from Plant Cells

    The isolation of mitochondria from plant cells that display similar biochemical

    2025-02-18 70
  • S期促进因子(S phase-promoting factor,SPF)

    S期促进因子(S phase-promoting factor,SPF)

    与MPF相似,芽殖酵母的S期促进因子(S phase-promoting factor,SPF),也是异质二聚体, 一个是Cdc28, 另一个是在G1期起作用的

    2025-02-18 46
  • PEG介导的动物细胞融合技术

    PEG介导的动物细胞融合技术

    相关专题 细胞融合(cell fusion)或细胞杂交(cell hybridization)是指真核细胞通过介导和培养,两个或多个细胞合并成一个双核或多核细

    2025-02-18 91
  • 细胞分泌(cell secretion)

    细胞分泌(cell secretion)

    动物细胞和植物细胞将在粗面内质网上合成而又非内质网组成部分的蛋白和脂通过小泡运输的方式经过高尔基体的进一步加工和分选运送到细胞内相应结构、细胞质膜以及细胞外的过

    2025-02-18 65
  • Examination of the Brain Mitochondrial Lipidome Using Shotgun Lipidomics

    Examination of the Brain Mitochondrial Lipidome Using Shotgun Lipidomics

    Contamination from subcellular organelles and myelin has hindered attempts to ch

    2025-02-18 60
  • Dorsal Axis Duplication as a Functional Readout for Wnt Activity

    Dorsal Axis Duplication as a Functional Readout for Wnt Activity

    The easy accessibility, distinctive features of early cleavage stage embryos and

    2025-02-18 52
  • High-Throughput FACS Method for Directed Evolution of Substrate Specificity

    High-Throughput FACS Method for Directed Evolution of Substrate Specificity

    Directed evolution is a powerful technology for the isolation of enzyme variants

    2025-02-18 64