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  • Site-Directed Mutagenesis Using a Rapid PCR-Based Method

    Site-Directed Mutagenesis Using a Rapid PCR-Based Method

    Site-directed mutagenesis is one of the fundamental tools available to probe the

    2025-02-18 46
  • Analyzing how Cell Adhesion Controls Mammary Gland Function by Transplantation of Embryonic Mammary

    Analyzing how Cell Adhesion Controls Mammary Gland Function by Transplantation of Embryonic Mammary

    Understanding how cell adhesion to the extracellular matrix controls mammalian d

    2025-02-18 50
  • Tips and Tricks for mFISH

    Tips and Tricks for mFISH

    Genetic analysis has been vastly improved by the introduction of the fluorescenc

    2025-02-18 46
  • Purification and Characterization of Poly(ADP-Ribosyl)ated DNA Replication/Repair Complexes

    Purification and Characterization of Poly(ADP-Ribosyl)ated DNA Replication/Repair Complexes

    PARP-1, the best studied isoform and most abundantly expressed member of the PAR

    2025-02-18 48
  • FISH Targeting of Chromosomes and Subchromosomal Regions in Yeast

    FISH Targeting of Chromosomes and Subchromosomal Regions in Yeast

    Yeasts represent powerful genetic model organisms, due in part to their tiny gen

    2025-02-18 54
  • High-Resolution NMR of DNA and Drug-DNA Interactions

    High-Resolution NMR of DNA and Drug-DNA Interactions

    The advantage of NMR over most other spectroscopic techniques lies in the abilit

    2025-02-18 52
  • Derivation and Characterization of Gut-Like Structures From Embryonic Stem Cells

    Derivation and Characterization of Gut-Like Structures From Embryonic Stem Cells

    Embryonic stem (ES) cells have a pluripotent ability to differentiate into a var

    2025-02-18 51
  • Percent Labeled Mitosis Curve Analysis

    Percent Labeled Mitosis Curve Analysis

    The measurement of percent labeled mitoses (PLM, or alternatively, FLM, for frac

    2025-02-18 47
  • Electroporation-Mediated Gene Transfer into Hepatocytes

    Electroporation-Mediated Gene Transfer into Hepatocytes

    The study of gene expression regulation relies on the introduction of foreign DN

    2025-02-18 56
  • Synthesis and Use of Spin-Labeled Lipids for Studies of the Transmembrane Movement of Phospholipids

    Synthesis and Use of Spin-Labeled Lipids for Studies of the Transmembrane Movement of Phospholipids

    The first measurement of the transmembrane diffusion of phospholipids in membran

    2025-02-18 58
  • 细胞生物基本方法:神经组织细胞培养

    细胞生物基本方法:神经组织细胞培养

    神经组织细胞培养1. 获取脑组织后,先仔细剥除脑膜和血管等纤维成分,置入 Hanks 液中漂洗 1 ~ 2 次后,置于 30 ~ 50 倍的 Hanks 液中,

    2025-02-18 57
  • Antisense-Mediated Inhibition of Protein Synthesis: Rational Drug Design, Pharmacokinetics, Intracer

    Antisense-Mediated Inhibition of Protein Synthesis: Rational Drug Design, Pharmacokinetics, Intracer

    There is hardly any class of drugs for which the term “ratronal drug design” is

    2025-02-18 48
  • Establishment and Maintenance of In Vitro Cultures of Human Retinal Pigment Epithelium

    Establishment and Maintenance of In Vitro Cultures of Human Retinal Pigment Epithelium

    The retinal pigment epithelium (RPE) is a layer of multipotential cells of neura

    2025-02-18 49
  • Protein Extraction for 2DE

    Protein Extraction for 2DE

    Our protein extraction protocol for two-dimensional gel electrophoresis (2DE) wa

    2025-02-18 50
  • Freezer and Cell Line Database

    Freezer and Cell Line Database

    Very useful Microsoft Access database program (486kb) for managing your cell lin

    2025-02-18 39