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培养物的污染及防止
相关专题 按现代的观念，凡是混入培养环境中对细胞生存产生有害的成分和造成细胞不纯的异物都应该视为污染。根据这一概念，组织培养污染物应包括生物（真菌、细菌 、病
2025-01-14 18 -
The Tight Junction, Intercellular Seal as a Cell Signaling Player: Protocols for Examination of Its
Tight junctions (TJs) are intercellular structures in epithelial and endothelial
2025-01-14 17 -
Molecular Methods Used for Detection of Minimal Residual Disease Following Hematopoietic Stem Cell T
Monitoring of minimal residual disease (MRD) in patients with acute or chronic m
2025-01-14 17 -
Stress Response Profiles in Human Fibroblasts Exposed to Heat Shock or Oxidative Stress
The upregulation of stress proteins is an important step in the cellular defense
2025-01-14 14 -
体外肝细胞损伤模型建立（CCl4和H2O2）
1 大鼠肝细胞的分离和培养 大鼠4％戊巴比妥麻醉，门静脉插管，先以无钙灌流液灌流，继以37℃通入O2的Ⅳ型胶原酶灌流液继续循环灌流15 min。将肝脏移至一平皿
2025-01-14 14 -
Lentiviral Vector-Mediated Gene Transfer in Embryonic Stem Cells
The major limitations in gene transduction to embryonic stem (ES) cells are (1)
2025-01-14 11 -
In Vitro Assays for Neural Stem Cell Differentiation
Neural stem cells (NSCs) are an undifferentiated population of cells residing in
2025-01-14 16 -
Rapid and Systematic Transfer and Recovery of Large BACs/PACs into Mammalian Cells by HAEC Retrofitt
Studies of the human genome have prompted the development of several cloning sys
2025-01-14 15 -
A Transplant Model for Human Epidermal Skin Regeneration
This protocol describes a technically simple in vivo assay of long-term skin reg
2025-01-14 11 -
Real-Time RT-PCR for CTG Repeat-Containing Genes
Myotonic dystrophy (DM1) is a neuromuscular disorder caused by a CTGn expansion
2025-01-14 18 -
Isolation of Total RNA from Bacteria
The susceptibihty of RNA to degradation by exogenous and endogenous RNase activi
2025-01-14 18 -
The Analysis of Mitochondria and Mitochondrial DNA in Human Embryonic Stem Cells
As human embryonic stem cells (hESCs) undergo differentiation, they express gene
2025-01-14 16 -
支原体污染后的治疗和处理
第一种方法：使用泰乐菌素（tylosin），Sigma，120多块钱 泰乐菌素是一种兽用抗生素,常用在鸡、猪的食料辅料，可以防止支原体感染引起的支气管哮喘，至今
2025-01-14 12 -
Quantitating mRNAs with Relative and Competitive RT-PCR
Reverse transcription coupled with the polymerase chain reaction (RT-PCR), permi
2025-01-14 14 -
Reverse Transfected Cell Microarrays in Infectious Disease Research
Several human pathogenic viruses encode large genomes with often more than 100 g
2025-01-14 10
