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Examining Planar Cell Polarity in the Mammalian Cochlea
The mammalian cochlea offers a unique opportunity to study the effects of planar
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Characterization of Cross-Linked Actin Filament Gels and Bundles Using Birefringence and Polarized L
Fundamental processes in the life of Dictyostelium , such as locomotion, endocyt
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Assay of Deoxyhypusine Hydroxylase Activity
The eukaryotic translation initiation factor 5A (eIF5A) is the only cellular pro
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Intravital Two-Photon Imaging of Adoptively Transferred B Lymphocytes in Inguinal Lymph Nodes
Intravital two-photon imaging allows the observation of immune cells in intact o
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Integration of Protein Kinases into Transcription Complexes: Identifying Components of Immobilised I
Regulation of gene expression is essential for coordinated cell growth and devel
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The Use of Electrophoretic Mobility Shift Assays to Study DNA Repair
DNA repair pathways must include proteins that recognize and bind to damaged DNA
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Determination of the Activity of Rho-Like GTPases in Cells
Rho-like GTPases, including RhoA, Rac1 and Cdc42, have been implicated in the co
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Methods to Assess Tissue Permeability
An essential requirement for adequate organ performance is the formation of perm
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自体组装(self assembly)
生物大分子借助本身的力量自行装配成高级结构,现代的概念应理解为不需要模板和酶系的催化, 以别于模板组装和酶效应组装。其实,这种组装也需要一种称为分子伴侣的蛋白介
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核糖体
核糖体不是由生物膜构成的,它是由蛋白质和RNA 构成的复合体。由大小两个亚基组成。核糖体是蛋白质合成的场所。附着在内质网上的核糖体合成的蛋白质主要有两类:一类是
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Nucleotide Metabolism and Cell-Cell Interactions
Interactions between the vascular endothelium and polymorphonuclear leukocytes (
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FISH on Sperms, Spermatocytes and Oocytes
It is well known that chromosome in situ hybridization allows the unequivocal id
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细胞转染方法
物理方法 电穿孔(electroporition) 显微注射(microinjection) 化学方法 磷酸钙转染(calcium phosphate tran
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Tissue Culture Media
Tissue Culture MediaWe use two different kinds of media. Most cells are grown in
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减数分裂
有性生殖的生物在生殖细胞形成前的某个时期进行的一种特殊方式的有丝分裂,其二倍体原始生殖细胞内染色体复制一次之后,细胞连续分裂两次,使生殖细胞中染色体数目比原始
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