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Purification of U Small Nuclear Ribonucleoprotein Particles
The spliceosome is the catalytic entity that removes the introns from the primar
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细胞化学染色分类技术
相关专题 染色技术是分类白细胞的经典依据,但瑞氏染色这类形态学复合染色不能被自动分析所模拟。一般光学技术只能测到光强等量的参数,而不能测得波长等属性的参数
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An Improved Rapid Method of Isolating RNA from Cultured Cells
The purification of good-quality RNA from tissue-cultured cells is essential for
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Cell-Cell Interactions on Solid Matrices
Models to study molecular, biochemical, and functional responses in vitro genera
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Oligonucleotide-Directed Random Mutagenesis Using the Phosphorothioate Method
Oligonucleotide-directed mutagenesis has become the primary method for testing t
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Implication of the ERK Pathway on the Post-transcriptional Regulation of VEGF mRNA Stability
Vascular Endothelial Growth Factor-A (VEGF-A) is one of the most important regul
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Duplex Unwinding and RNP Remodeling With RNA Helicases
RNA helicases are essential for the adenosine 5′-triphosphate (ATP)-driven rearr
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In Vitro Delivery of Drugs and Other Molecules to Cells
The permeability of a cell membrane can be transiently increased locally when an
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Histone Modification Profiling in Normal and Transformed Human Embryonic Stem Cells Using Micro Chro
Comparing normal human embryonic stem cells (hESCs) to those that have acquired
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Glutaraldehyde-Mediated Protein Immobilization
In this chapter, we describe different approaches for the utilization of glutara
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Reprogramming of Liver to Pancreas
Islet grafts have demonstrated that patients with diabetes would benefit greatly
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Assays for Hypermethylation of the BRCA1 Gene Promoter in Tumor Cells to Predict Sensitivity to PARP
The breast cancer 1 and 2 , early onset (BRCA1 and BRCA2 ) genes are important f
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Genetic Reconstitution of Bone Marrow for the Study of Signal Transduction Ex Vivo
Introducing genes into cells by retroviral transduction has greatly increased th
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Preparation of tubulin
Although many protocols for tubulin preparation are available, the procedure des
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Automated Live Microscopy to Study Mitotic Gene Function in Fluorescent Reporter Cell Lines
Fluorescence live microscopy is a powerful technique to study complex cellular d
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