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细胞自噬：从基础到临床，生命科学的深度解析
一、细胞自噬的基础概念细胞自噬，简而言之，就是细胞自己吃自己。这一过程涉及溶酶体和自噬体的共同参与，通过形成自噬溶酶体来降解受损、衰老或过剩的生物大分子和细胞器
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Separation of Thylakoid Membrane Proteins by Sucrose Gradient Ultracentrifugation or Blue Native-SDS
Generally, a combination of two or more chromatographic and/or electrophoretic m
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Electrofusion of Mouse Embryos to Produce Tetraploids
It is possible to generate tetraploidy in mammalian embryos by chemical or physi
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Visualizing Signaling and Cell Movement During the Multicellular Stages of Dictyostelium Development
Time-lapse microscopy provides a powerful tool with which to study cell behavior
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多线染色体(polytene chromosome)
核内DNA多次复制产生的子染色体平行排列, 且体细胞内同源染色体配对, 紧密结合在一起, 从而阻止了染色体纤维进一步聚缩, 形成体积很大的由多条染色体组成的结构
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Immunofluorescence of Cultured Cells
The development and the extensive use of immunofluorescence microscopy on cultur
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DNA Damage Response and Mutagenesis in Mouse Embryonic Stem Cells
Mutation in embryonic stem (ES) cells can potentially compromise multiple cell l
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Tissue Procurement for Molecular Studies Using Laser-Assisted Microdissection
Properly collected and stored human specimens offer the unique opportunity to st
2025-01-14 11 -
Pharmacology of Antisense Therapeutic Agents: Cancer and Inflamination
Antisense oligonucleotides represent a new paradigm for drug discovery that hold
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Oligonucleotide-Directed Mutagenesis Using an Improved Phosphorothioate Approach
Oligonucleotide-directed in vitro mutagenesis is an established tool for the inv
2025-01-14 19 -
Generation of Nonviral Integration-Free Induced Pluripotent Stem Cells from Plucked Human Hair Folli
Human induced pluripotent stem cells (hiPSCs) provide a unique experimental reag
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Structural Studies of MAP Kinase Cascade Components
MAPK cascade components have been the subject of structural analysis, advancing
2025-01-14 10 -
RACE实验是什么？【分子生物学】
RACE实验的类型多样，包括经典RACE、Cap-switching RACE、环形RACE等。每种类型都有其独特的原理和应用场景，如经典RACE主要用于克隆c
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Preparation of DRV Liposomes
Dried reconstituted vesicles (DRV) are liposomes that are formulated under mild
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Procedures to Evaluate the Importance of Dietary Polyamines
Polyamines not only play vital physiological functions including modulating tran
2025-01-14 13
