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  • The Use of Retroviral Vectors for tet-Regulated Gene Expression in Cell Populations

    The Use of Retroviral Vectors for tet-Regulated Gene Expression in Cell Populations

    Today the treatment of inherited diseases holds a major field in gene therapy, a

    2025-02-14 47
  • Analysis of Ca2+-Binding Sites in the MthK RCK Domain by X-Ray Crystallography

    Analysis of Ca2+-Binding Sites in the MthK RCK Domain by X-Ray Crystallography

    Regulator of K+ conductance (RCK) domains form a conserved class of ligand-bindi

    2025-02-14 39
  • The Basic-Helix-Loop-Helix-Leucine Zipper Gene Mitf: Analysis of Alternative Promoter Choice and Spl

    The Basic-Helix-Loop-Helix-Leucine Zipper Gene Mitf: Analysis of Alternative Promoter Choice and Spl

    The activity of transcription factors is often regulated by Post-translational m

    2025-02-14 45
  • Histochemistry and Lipid Profiling Combine for Insights into Aging and Age-Related Maculopathy

    Histochemistry and Lipid Profiling Combine for Insights into Aging and Age-Related Maculopathy

    Aging is the major risk factor for age-related maculopathy (ARM), the biggest ca

    2025-02-14 41
  • Identification and Assay of Allosteric Regulators of S-Adenosylmethionine Decarboxylase

    Identification and Assay of Allosteric Regulators of S-Adenosylmethionine Decarboxylase

    Polyamine biosynthesis is extensively regulated in cells by multiple mechanisms,

    2025-02-14 48
  • Investigation of Genomic Methylation Status Using Methylation-Specific and Bisulfite Sequencing Poly

    Investigation of Genomic Methylation Status Using Methylation-Specific and Bisulfite Sequencing Poly

    Epigenetic modification plays a central role in the regulation of gene expressio

    2025-02-14 46
  • Variations on the Single-Molecule Assay for Microtubule-Associated Proteins and Kinesins

    Variations on the Single-Molecule Assay for Microtubule-Associated Proteins and Kinesins

    The direct observation of single kinesins and microtubule-associated proteins (M

    2025-02-14 41
  • 单克隆抗体的制备与鉴定方法

    单克隆抗体的制备与鉴定方法

    相关专题 生物帮之抗体制备摘要:从人脐静脉分离的内皮细胞经培养后免疫 Balb/c小鼠,利用小鼠杂交瘤技术,建立了一株持续分泌抗人内皮细胞单克隆抗体的杂交瘤。对

    2025-02-14 48
  • Derivation of Primary Mouse Embryonic Fibroblast (PMEF) Cultures

    Derivation of Primary Mouse Embryonic Fibroblast (PMEF) Cultures

    Primary mouse embryonic fibroblasts (PMEFs) have a number of properties that mak

    2025-02-14 49
  • Glomerular Epithelial and Mesangial Cell Culture and Characterization

    Glomerular Epithelial and Mesangial Cell Culture and Characterization

    The advent of in vitro culture techniques has enabled the culture of homogeneous

    2025-02-14 49
  • Matrix Assembly, Cell Polarization, and Cell Survival: Analysis of Peri-Implantation Development Wit

    Matrix Assembly, Cell Polarization, and Cell Survival: Analysis of Peri-Implantation Development Wit

    A variety of mutations, including those affecting laminin expression and basemen

    2025-02-14 48
  • Isolation and Purification Method of Mouse Fetal Hepatoblasts

    Isolation and Purification Method of Mouse Fetal Hepatoblasts

    During development, liver precursors constitute a valuable source of pluripotent

    2025-02-14 47
  • Genome-Wide Analysis of RNA Extracted from Isolated Mitochondria

    Genome-Wide Analysis of RNA Extracted from Isolated Mitochondria

    Isolating mitochondria by subcellular fractionation is a well-established method

    2025-02-14 46
  • Assays of Lipolytic Enzymes

    Assays of Lipolytic Enzymes

    Free fatty acids (FAs) derived from adipose tissue (AT) are, quantitatively, the

    2025-02-14 45
  • Preparing Mitochondria from Rat Liver

    Preparing Mitochondria from Rat Liver

    Liver is a convenient source for functional intact mitochondria for a number of

    2025-02-14 41