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细胞转染实验的方法都有什么？
1. 电穿孔法。通过短暂的高电场电脉冲处理细胞，沿细胞膜的电压差异会导致细胞膜的暂时穿孔。DN被认为是穿过孔扩散到细胞内的。电脉冲和场强的优化对于成功的转染非常
2025-01-09 19 -
细胞周期测定实验有哪些，怎么做?
细胞计数法实验方法原理：体外培养细胞生长、分裂繁殖的能力，可用分裂指数来表示。它与生长曲线有一定的联系，如随着分裂指数的不断提高，细胞也就进入了指数生长期。分裂
2025-01-09 30 -
如何分离成纤维细胞？
下述的通用操作方案适用于小鼠、大鼠、仓鼠和鸡胚胎。选择大约一半足孕时间的胚胎最好，10 ~ 13 天龄胚胎含有最大数量的未分化的间质细胞，成纤维细胞可从这些细胞
2025-01-09 17 -
siRNA转染后如何在蛋白水平敲低
可能的原因：蛋白降解滞后性：即使mRNA水平在48小时时已经显著下降，但蛋白的降解可能需要更长的时间。蛋白具有相对较长的半衰期，因此在mRNA水平下降之后，蛋白
2025-01-09 17 -
高滴度反转录病毒和慢病毒载体的制备
一、试剂CaCl2 溶液( 2mol/L )、完全培养基、DMEM 培养基、乙醇（或异丙醇） (70% )、胎牛血清( FBS )、HBS 溶液(2X)、HEP
2025-01-09 13 -
慢病毒感染后72小时，效率低，细胞已经长满，有什么办法补救？
在慢病毒感染后72小时感染效率较低、细胞已经长满的情况下，可以尝试以下几个措施来提高感染效率并优化实验条件：细胞传代或重新播种：如果细胞已经长满（汇合度较高），
2025-01-09 13 -
Behavioral Methods for the Study of the RasERK Pathway in Memory Formation and Consolidation: Passiv
Memory is a high-level brain function that enables organisms to adapt their beha
2025-01-08 17 -
Bone Marrow-Derived Mesenchymal Progenitor Cells
Primary monolayer cultures of marrow stromal cells, as they are referred to in t
2025-01-08 21 -
Skeletal Morphogenesis
The formation of a skeleton with its numerous bones of various shapes and sizes
2025-01-08 18 -
Function of the Wingless Signaling Pathway in Drosophila
Signaling by the wingless pathway has been shown to govern numerous developmenta
2025-01-08 15 -
Protein-Deoxyribonucleic Acid Interactions Linked to Gene Expression: DNase I Digestion
Mapping DNase I hypersensitive sites can often localize the control regions in a
2025-01-08 22 -
Retroviral and Lentiviral Vector Titration by the Analysis of the Activity of Viral Reverse Transcri
The assessment of the viral titer is an important step in optimizing and reprodu
2025-01-08 13 -
Developmental Genetics in Xenopus tropicalis
The diploid pipid frog Xenopus tropicalis has recently emerged as a powerful new
2025-01-08 17 -
Using 3D Culture to Investigate the Role of Mechanical Signaling in Keratinocyte Stem Cells
The ability to grow keratinocyte stem cells (KSCs) in 3D culture is an important
2025-01-08 17 -
Analysis of Replicating Mitochondrial DNA by Two-Dimensional Agarose Gel Electrophoresis
Replication intermediates can be separated on agarose gels in two dimensions to
2025-01-08 14
