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荧光表达的细胞在冻存后72小时后再测荧光情况,可以吗?
荧光表达后的细胞可以在冻存后72小时再测荧光,但有几点需要注意:细胞存活率:冻存和解冻过程可能会影响细胞的存活率和状态。确保细胞在解冻后恢复良好,具有正常的形态
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双脱氧法DNA 测序常见问题
问题可能原因解决方法放射自显影片空白DNA 聚合酶失活标记物太旧用对照引物、模板及试剂进行试验试剂太旧替换缺陷试剂不正确或有缺陷的引物不正确或有缺陷的模板,曝光
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Quantification of Genetically Controlled Cell Death in Budding Yeast
Yeast are the foremost genetic model system. With relative ease, entire chemical
2025-01-07 11 -
The Isolation of Osteoclasts from Human Giant Cell Tumors and Long-Term Marrow Cultures
The osteoclast is a large multinucleate cell formed from the fusion of mono-nucl
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细胞生物基本方法:特殊培养法
特殊培养法 1 )二倍体细胞培养法 二倍体细胞培养法与一般培养相同,关键在于传代,其传代程序为:1. 吸除旧培养液注入另瓶中。2. 用温 B
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TUNEL PROCEDURE IN BOVINE EMBRYOS
Materials8% (w/v) paraformaldehyde stock solution: Dissolve 8 g of powdered pa
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Electrofusion of Mammalian Cells
Electrofusion has developed into an extremely efficient method for the fusion of
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Nuclear Reprogramming: An Overview
Nuclear reprogramming is an intriguing phenomenon, in which specialized somatic
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双线期(diplotene stage)
前期I的第四个阶段,此期染色体长度进一步变短,联会复合体因发生去组装而逐渐消失,紧密配对的同源染色体相互分开,而在非姊妹染色单体之间的某些部位上,可见其相互间有
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Analysis of Tumor and Endothelial Cell Viability and Survival Using Sulforhodamine B and Clonogenic
A variety of assays, and rationales for their use, exist to monitor viability an
2025-01-07 13 -
Construction of Polycompetitors for Competitive PCR
Many different protocols are now available for competitive polymerase chain reac
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A Rapid and Efficient Platelet Purification Protocol for Platelet Gene Expression Studies
Isolation of pure platelet samples from whole blood is crucial for the study of
2025-01-07 12 -
Electrochemotherapy for the Treatment of Soft Tissue Sarcoma in a Rat Model
Each year approximately 6000 new cases of soft tissue sarcoma are diagnosed in t
2025-01-07 12 -
Analysis of ES Cell Clones by Mini-Southern
Analysis of ES Cell Clones by Mini-SouthernInitial Cloning 1. Aspirate selective
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Organelle Proteomics
The proteome of the cell is at the frontier of being too complex for proteomic a
2025-01-07 16