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Cell-Cell Interaction in the Transcellular Biosynthesis of Novel -3-Derived Lipid Mediators
Omega-3 polyunsaturated fatty acids (PUFAs) such as eicosapentaenoic acid (EPA)
2025-01-06 12 -
Pseudo-Immunolabelling With the AvidinBiotinPeroxidase Complex due to the Presence of Endogenous Bio
Immunodetection techniques are dependent on enzyme–protein conjugates for the vi
2025-01-06 11 -
Studies on Carbohydrate Xenoantigens
Naturally occurring and elicited anti-carbohydrate antibodies play a major role
2025-01-06 13 -
Amplification of Fungal Genomes Using Multiple Displacement Amplification
The availability of genomic DNA of sufficient quality and quantity is fundamenta
2025-01-06 11 -
Differentiation of Human Embryonic and Induced Pluripotent Stem Cells into Blood Cells in Coculture
The establishment of human embryonic stem cell (hESC) lines, as well as the rece
2025-01-06 10 -
Ultraviolet-Induced Apoptosis in Embryonic Stem Cells In Vitro
Embryonic stem (ES) cells, and the inner cell mass from which they are derived,
2025-01-06 15 -
Automation of Fluorescent Differential Display With Digital Readout
Since its invention in 1992, differential display (DD) has become the most commo
2025-01-06 13 -
Determination of Nuclear Localization Signal Sequences for Krppel-Like Factor 8
Transcription factor proteins function in the nucleus to regulate gene expressio
2025-01-06 14 -
FISH and Immunofluorescence Staining in Chlamydomonas
Here we describe how to use fluorescence in situ hybridization and immunofluores
2025-01-06 13 -
Preparation of low density, collagenase-digested splenocytes Protocol
Preparation of low density, collagenase-digested splenocytes Protocol 1. Dilute
2025-01-06 11 -
Isolation, Characterization, and Culture of Epithelial Stem Cells
It is well accepted that homeostasis of continuously renewing adult tissues, suc
2025-01-06 20 -
Tissue-Specific KO of ECM Proteins
The analysis of phenotypes caused by null and mutant alleles is a very powerful
2025-01-06 22 -
In Vitro Replication Assay with Mammalian Cell Extracts
Regulatory mechanisms for DNA replication are crucial to the control of the cell
2025-01-06 21 -
常用的蛋白水解酶抑制剂
当细胞被裂解，内容物释放时，蛋白水解酶和其他一些降解的酶也一同被释放，这时候需要在裂解的缓冲液中加入蛋白水解酶。PS: 细胞自己的蛋白水解酶也会降解细胞内的蛋白
2025-01-06 25 -
RNA 凝胶电泳注意事项
RNA 凝胶是用Northern 印迹的方法来分析RNA 。mRNA 大约只占总RNA 的5% ，在溴化乙锭染色的胶上看不到，因此mRNA 必须用标记探针来检测
2025-01-06 23
