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Detection of the Nuclear Poly(ADP-ribose)-Metabolizing Enzymes and Activities in Response to DNA Dam
Poly(ADP-ribosyl)ation is a posttranslational modification of proteins in higher
2025-01-06 19 -
Production of Membrane Proteins in Escherichia coli and Lactococcus lactis
As the equivalent to gatekeepers of the cell, membrane transport proteins perfor
2025-01-06 22 -
Automated Video Image Analysis of Larval Zebrafish Locomotor Rhythms
A method is described for measurement of the circadian activity rhythms of up to
2025-01-06 21 -
Raman Microscopy of Bone
Raman microscopy is a non-destructive technique requiring minimal sample prepara
2025-01-06 11 -
Detection of Terminal Transferase in Leukemia
The mere presence of terminal deoxynucleotidyl transferase (TdT), a DNA polymera
2025-01-06 18 -
In Vivo Assay for Tumor Cell Invasion
We describe an in vivo invasion assay that enables the collection of invasive ce
2025-01-06 22 -
Detection of SPO11-Oligonucleotide Complexes from Mouse Testes
The SPO11 protein generates programmed DNA double-strand breaks (DSBs) that init
2025-01-06 14 -
Stable Isotope Labelling with Amino Acids in Cell Culture for Human Embryonic Stem Cell Proteomic An
The identification and quantitative measurements of proteins in human embryonic
2025-01-06 15 -
MT Spindowns from Extracts
Notes:The key variable in MT spindown experiments is ATP. Under high ATP conditi
2025-01-06 14 -
Retroviral Gene Transfer into Primary Human Natural Killer Cells
Modulation of intracellular signaling pathways or receptor expression in natural
2025-01-06 22 -
Isolation and Analysis of Lipid Rafts in CellCell Interactions
Lipid rafts are dynamic structures made up of proteins and lipids that float fre
2025-01-06 33 -
Differentiation of Embryonic Stem Cells to Retinal Cells In Vitro
Currently, there is no effective treatment for photoreceptor degeneration, the m
2025-01-06 15 -
Blood Cell Capture on Antibody Microarrays and Monitoring of the Cell Capture Using Surface Plasmon
Blood is a tremendous source of data for diagnostic purposes. Thanks to the qual
2025-01-06 15 -
Coimmobilization of Enzymes and Cells
Coimmobilization of cells and/or enzymes is performed for three main reasons: fi
2025-01-06 14 -
The Generation of Embryoid Bodies from Feeder-Based or Feeder-Free Human Pluripotent Stem Cell Cultu
Embryoid body (EB) formation is a traditional method of inducing differentiation
2025-01-06 16