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微管组织中心(microtubule organizing centers, MTOC)
存在于细胞质中决定微管在生理状态或实验处理解聚后重新组装的结构叫微管组织中心。在多数情况下MTOC有一对中心粒和一个中心体, 但是某些表皮细胞和新受精的卵细胞，
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相差观察和摄影
相差观察和摄影 1） 相差装置的使用1． 先调好相位板，使聚光器相位板号与接物镜放大倍数相一致。2． 然后抽出接目镜，再换辅助望远镜，移动辅助镜筒并调整聚光器
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Use of Gene Overexpression to Assess Function in Cell Cycle Control
Transient overexpression of wild-type or mutant cell cycle regulatory factors is
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Using an Inverse PCR Strategy to Clone Large, Contiguous Genomic DNA Fragments
Conventional PCR screens of genomic DNA will often yield a substantial fragment
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Cell Cycle Analysis of Asynchronous Populations
Measurement of a DNA histogram can be achieved by fixing or permeabilizing cells
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Freeze-Fracture Electron Microscopy on Domains in Lipid Mono- and Bilayer on Nano-Resolution Scale
Freeze-fracture electron microscopy (FFEM) as a cryo-fixation, replica, and tran
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Methods for Determining the Proliferation of Cells in Response to EGFR Ligands
The evaluation of cell proliferation can be accomplished by several methods. The
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Splice Redirection as a Convenient Assay to Monitor CPPON Efficiency and Mechanism
Several strategies based on synthetic oligonucleotides (ON) have been proposed t
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Obtaining Freshly Isolated and Cultured Mesenchymal Stem Cells from Human Adipose Tissue
The stromal compartment of adipose tissue harbors mesenchymal stem cells (MSCs)
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The Reconstitution of Actin Polymerization on Liposomes
Membrane-associated actin polymerization is of considerable interest due to its
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Measuring Angiogenesis in Mice
Angiogenesis is the formation of new blood vessels from pre-existing vessels. Th
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Peptidomic Analysis of Single Identified Neurons
Today, commercially available mass spectrometers increasingly meet all the deman
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圆球体(spherosome)
植物细胞中由一层单位膜包裹的含有细微结构的球形颗粒，直径为0.5～1μm，内含酸性水解酶，相当于动物细胞的溶酶体。与动物细胞的溶酶体不同，圆球体能够被脂溶性的染
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FISH Methods in Cytogenetic Studies
This chapter describes the various methods derived from the protocol of standard
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Techniques for Studying the Electrophysiology of Neurons Derived from Neural Stem/Progenitor Cells
The use and study of neural stem cells (NSCs) both in vitro and in vivo has incr
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