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原代培养(primary culture)
原代培养是指直接从机体取下细胞、组织和器官后立即进行培养。因此，较为严格地说是指成功传代之前的培养，此时的细胞保持原有细胞的基本性质，如果是正常细胞，仍然保留二
2025-01-03 15 -
Basic Approaches to Gene Expression Analysis of Stem Cells by Microarrays
This chapter covers gene expression analysis by microarray to study and characte
2025-01-03 13 -
Design and Production of Retro- and Lentiviral Vectors for Gene Expression in Hematopoietic Cells
Successful retroviral gene transfer into hematopoietic cells has been demonstrat
2025-01-03 15 -
In Vitro Fabrication of Engineered Human Skin
In vitro fabrication of human epidermal tissues that mimic the biochemical and m
2025-01-03 28 -
Using Somatic-Cell Nuclear Transfer to Study Aging
In mammals, a diploid genome following fertilization of haploid cells, an egg, a
2025-01-03 14 -
Optimized Protocols to Analyze Sphingosine-1-Phosphate Signal Transduction Pathways During Acrosomal
Regulated secretion is a central issue for the specific function of many cells;
2025-01-03 15 -
Immunosubtraction as a Means of Typing Monoclonal and Other Proteins in Serum and Urine
Serum and urine protein electrophoresis are used primarily to screen for the pre
2025-01-03 10 -
Three-Dimensional Alignment of Schwann Cells Using Hydrolysable Microfiber Scaffolds: Strategies for
Injuries to the peripheral nervous system affect 1 in 1,000 individuals each yea
2025-01-03 11 -
Solid Tumor DNA Ploidy Analysis
Flow cytometric DNA content analysis of human neoplasia provides quantitative in
2025-01-03 20 -
Pheromone Halo Assay
Pheromone Halo Assay REFERENCE: Hoffman, G., Garrison, T. R., and Dohlman, H. G.
2025-01-03 17 -
Assaying Phosphoinositide Phosphatases
The roles of phosphoinositide second messengers as signaling molecules in a vast
2025-01-03 9 -
Array-Comparative Genomic Hybridization Characterization of Human Pluripotent Stem Cells
During culture adaptation, human embryonic stem cells (ESCs) and induced pluripo
2025-01-03 11 -
细胞培养――细胞生长和细胞毒性
Articles posted in the Method FroumCell Viability Assay Dye exclusion methodViab
2025-01-03 16 -
Transport of Antisense Across the Blood-Brain Barrier
Antisense-mediated therapy holds great promise for the treatment of central nerv
2025-01-03 12 -
Ier1 p: A Kinase and Site-Specific Endoribonuclease
The lumen of the endoplasmic reticulum (ER) is a highly specialized compartment
2025-01-03 19
