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大鼠嵌合体制备技术-聚集法制作嵌合体大鼠
简介嵌合体(chimera)在遗传学上用于指不同遗传性状嵌合或混杂表现的个体;在免疫学上的涵义则指一个机体身上有两种或两种以上染色体组成不同的细胞系同时存在,彼
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新生儿缺氧缺血性脑病动物模型的建立
简介建立 HIBD 动物模型的经典方法是 Rice-Vannucci 模型,大鼠脑的血液供应与人类相似,颈内动脉和椎动脉在大脑底部形成 Willis 环。在研究
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Cell Labeling and Gene Misexpression by Electroporation
Recent advances in molecular biology have led to the identification of several g
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Methods to Detect Patterns of Cell Death in Drosophila
There are many times during Drosophila development or in the adult when cell dea
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Fluorescent In Situ Hybridization Protocols in Drosophila Embryos and Tissues
Fluorescent in situ hybridization is the standard method for visualizing the spa
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The Use of Isothermal Titration Calorimetry to Study Multidrug Transport Proteins in Liposomes
Biophysical measurements of multidrug transporters in vitro can often be of limi
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Assaying Phospholipase A2 Activity
Mammalian cells contain many structurally and functionally diverse phospholipase
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Analyzing p53 Regulated DNA Damage Checkpoints by Flow Cytometry
The most critical feature of the cellular response to DNA damage is the ability
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Microarray-CGH for the Assessment of Aneuploidy in Human Polar Bodies and Oocytes
The cytogenetic analysis of single cells, such as oocytes and polar bodies, is e
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Permeability of Differentiated Human Urothelium In Vitro
The urothelium plays a critical role in the bladder as a permeability barrier to
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The Use of Permeabilized Cell Systems to Study Nuclear Transport
Nucleocytoplasmic transport occurs through nuclear pore complexes (NPC), macromo
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Fluorescence Correlation Spectroscopy for the Study of Membrane Dynamics and Organization in Giant U
Fluorescence correlation spectroscopy (FCS) is a powerful technique to study the
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Sample Preparation for Fluorescence Imaging of the Cytoskeleton in Fixed and Living Plant Roots
During the past decade the use of live cytoskeletal probes has increased dramati
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Genetic and Biochemical Analysis of Protozoal Polyamine Transporters
Polyamines are aliphatic polycations that function in key cellular processes suc
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In Vitro Nanoparticle-Mediated Intracellular Delivery into Human Adipose-Derived Stem Cells
Adipose-derived stem cells (ASCs) are multipotent cells that are emerging as an
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