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Culture of Adipose Tissue and Isolated Adipocytes
Adipose tissue (AT) function is regulated by both short-term and long-term mecha
2025-01-03 13 -
Development of Multiple-Well Plate Biological Assays
This chapter focuses on the application of multiple-well plate technology to bio
2025-01-03 12 -
Detection of Rhythmic Bioluminescence From Luciferase Reporters in Cyanobacteria
The unicellular cyanobacterium Synechococcus elongatus PCC 7942 is the model org
2025-01-03 10 -
Keeping Two Animal Systems in One Lab A Frog Plus Fish Case Study
For two decades, my lab has been studying development using two vertebrate anima
2025-01-03 10 -
Expression and Purification of Membrane-Type MMPs
Matrix metalloproteinases (MMPs) modulate cell–cell and cell–matrix interactions
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Chimeric Animals and GermlineTransmission
Gene targeting by homologous recombination in pleuripotent embryonic stem (ES) c
2025-01-03 10 -
Creating 2DE Databases for the World Wide Web
With the development of the Internet, a growing number of two-dimensional electr
2025-01-03 10 -
Interspecific Chimeras in Avian Embryos
Avian embryos provide all sorts of opportunities for the study of higher vertebr
2025-01-03 11 -
Environmental Scanning Electron Microscope Imaging of Vesicle Systems
The structural characteristics of liposomes have been widely investigated and th
2025-01-03 10 -
Drosophila melanogaster as a Model System to Study Mitochondrial Biology
Mitochondria play an essential role in cellular homeostasis. Although in the las
2025-01-03 12 -
Methodology of Aniline Blue Staining of Chromatin and the Assessment of the Associated Nuclear and C
In this chapter, the laboratory methods for detection of sperm biomarkers that a
2025-01-03 13 -
Mitochondrial Fusion In Vitro
The field of mitochondrial dynamics has received a great deal of attention as a
2025-01-03 11 -
Enzyme-Linked Immunosorbent Assay for Screening of Antibodies in Hybridoma Supernatants
Enzyme-linked immunosorbent assay (ELISA) is a rapid and convenient method for s
2025-01-03 13 -
The Use of JAK-Specific Inhibitors as Chemical Biology Tools
The JAK family of protein tyrosine kinases are now recognized as important parti
2025-01-03 11 -
Neural Crest Cell Outgrowth Cultures and the Analysis of Cell Migration
The neural crest gives rise to many differentiated cell types including craniofa
2025-01-03 11