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  • Electron Spectroscopic Imaging of the Nuclear Landscape

    Electron Spectroscopic Imaging of the Nuclear Landscape

    Our understanding of sub-nuclear organisation is largely based on fluorescence a

    2025-02-06 47
  • A Rapid Tracking Method for the Quantitative Analysis of Organelle Streaming Velocity

    A Rapid Tracking Method for the Quantitative Analysis of Organelle Streaming Velocity

    A key to understanding cytoskeletal mechanisms of eukaryotic cells is found in t

    2025-02-06 41
  • Isolation of Novel +TIPs and Their Binding Partners Using Affinity Purification Techniques

    Isolation of Novel +TIPs and Their Binding Partners Using Affinity Purification Techniques

    Microtubule organization and dynamics are controlled by a large set of cellular

    2025-02-06 46
  • StemBase: A Resource for the Analysis of Stem Cell Gene Expression Data

    StemBase: A Resource for the Analysis of Stem Cell Gene Expression Data

    StemBase is a database of gene expression data obtained from stem cells and deri

    2025-02-06 43
  • Preparation of Hela Cell Nuclear and Cytosolic S100 Extracts for In Vitro Splicing

    Preparation of Hela Cell Nuclear and Cytosolic S100 Extracts for In Vitro Splicing

    Following the initial discovery of split genes in 1977, it took several years be

    2025-02-06 36
  • Methods for Analyzing MicroRNA Expression and Function During Osteogenic Differentiation of Human Ad

    Methods for Analyzing MicroRNA Expression and Function During Osteogenic Differentiation of Human Ad

    MicroRNAs (miRNA) are single-stranded RNA molecules of 21–23 nucleotides in leng

    2025-02-06 47
  • Methylation Profiling Using Methylated DNA Immunoprecipitation and Tiling Array Hybridization

    Methylation Profiling Using Methylated DNA Immunoprecipitation and Tiling Array Hybridization

    DNA methylation is an important epigenetic modification that regulates developme

    2025-02-06 47
  • Culture of Human Pluripotent Stem Cells on Glass Slides for High-Resolution Imaging

    Culture of Human Pluripotent Stem Cells on Glass Slides for High-Resolution Imaging

    For certain applications, particularly experiments involving high-resolution ima

    2025-02-06 46
  • 亮氨酸拉链基元(leucine zipper motif)

    亮氨酸拉链基元(leucine zipper motif)

    这类蛋白质都是含有4个或5个亮氨酸残基, 彼此之间精确的相距7个氨基酸残基。这样, 在α螺旋的每一个侧面就出现一个Leu, 这些Leu排成一排, 两个蛋白质分子

    2025-02-06 53
  • Detection of Apoptosis in Drosophila

    Detection of Apoptosis in Drosophila

    Drosophila has unique genetic and cell biological advantages as a model system f

    2025-02-06 46
  • Analysis of HOX Homeodomain Proteins and Gene Transcripts in the Epidermis

    Analysis of HOX Homeodomain Proteins and Gene Transcripts in the Epidermis

    HOX homeodomain proteins are thought to be master developmental regulators of ti

    2025-02-06 39
  • The Use of Real-Time Quantitative PCR for the Analysis of Cytokine mRNA Levels

    The Use of Real-Time Quantitative PCR for the Analysis of Cytokine mRNA Levels

    Over the last decade, real-time-quantitative PCR (RT-qPCR) analysis has become t

    2025-02-06 45
  • Solid-Phase Screening Using Digital Image Analysis

    Solid-Phase Screening Using Digital Image Analysis

    It is often possible to screen libraries of variant enzymes directly on agar pla

    2025-02-06 52
  • Practical Protocols for Lipase Immobilization via SolGel Techniques

    Practical Protocols for Lipase Immobilization via SolGel Techniques

    Lipases can be efficiently entrapped in the pores of hydrophobic silicates by a

    2025-02-06 55
  • Methods for Studying Activation of Matrix Metalloproteinases

    Methods for Studying Activation of Matrix Metalloproteinases

    The degradation of the extracellular matrix during development and in disease is

    2025-02-06 49